超高效液相色谱-二极管阵列检测器检测中药散剂中磺胺类药物  

Determination of Sulfonamides in Traditional Chinese Medicine Powder by UPLC-DAD

作　　者：刘素梅[1] 韩立[1] 方忠意[1] 李华岑[1] 王丽[2] 张跃京[1] 臧合英[1] 

机构地区：[1]河南省兽药监察所,郑州450008 [2]郑州大学药学院,郑州450001

出　　处：《中国兽药杂志》2011年第10期28-30,33,共4页Chinese Journal of Veterinary Drug

摘　　要：建立了中药散剂中非法添加磺胺类药物检测的超高效液相色谱法。样品经甲醇提取、流动相稀释后进高效液相色谱仪进行检测分析。色谱条件:ACQUITY UPLC HSS T3(1.8μm,2.1×100 mm)色谱柱,流动相为乙腈-0.017 mol/L的磷酸溶液系统,检测波长范围200～340 nm,柱温30℃,进样量为3μL,磺胺类药物在0.2～200μg/mL范围内呈良好的线性关系。该方法简便,准确,通过光谱比较,可以准确地判断中药中是否添加有磺胺类药物并进行准确地定量计算。A method of UPLC-PDA for determination of sulfonamides in traditional Chinese medicine was developed.The liquid chromatography separation was carried on ACQUITY UPLC HSS T3（1.8 μm,2.1×100 mm）with acetonitrile-0.017 mol/L H3PO4 system as mobile phase.The wavelength range was 190~400 nm.The calibration curve was linear in the range of 0.2~200 μg/mL.The method was simple,and would accurately detect sulfonamides in traditional Chinese medicine powder.

关 键 词：中药散剂 磺胺类药物 超高效液相色谱 二极管阵列检测器 

分 类 号：S853.7[农业科学—临床兽医学]