女生初潮年龄提前与黄体生成素受体基因Asp^(578)Gly突变  

Asp^(578) Gly mutation of luteinizing hormone receptor in girls with early menarche

作　　者：朱丁[1,2] 钱红丹[1] 徐勇[3] 张敏婕[3] 沈惠芬 刘起展[1] 

机构地区：[1]南京医科大学公共卫生学院,江苏210029 [2]无锡市疾病预防控制中心 [3]苏州大学医学部公共卫生学院

出　　处：《中国学校卫生》2011年第9期1028-1029,1031,共3页Chinese Journal of School Health

基　　金：国家自然基金项目(81072312);无锡市卫生局指导性课题(ZD0901)

摘　　要：目的研究黄体生成素受体(LHR)基因Asp578Gly突变与女生初潮年龄提前的关系,为青少年青春期发育研究提供依据。方法分层整群抽取无锡市177名初潮年龄提前(初潮年龄<11岁)女生,外周血提取DNA,用5'-CAC TGC TGG CTT TTT CAC TGTATT-3'和5'-TGA AGG CAG CTG AGA TGG CAA AAA-3'作引物,经常规PCR扩增后,用限制性内切酶MspⅠ消化,以检测LHR基因是否存在A1733G的碱基突变。结果初潮年龄提前组LH水平高于正常组(P<0.05),而两组FSH差异无统计学意义(P>0.05)。177名初潮年龄提前女生的LHR基因均未检测到Asp578Gly突变。结论 Asp578Gly突变与女性初潮年龄提前无直接关系。Objective To identify the relationship between early menarche and Asp57s Gly mutation of luteinizing hormone receptor（ LHR）. Methods A total of 177 girls defined as early menarche （ age of menarehe less than 11 years old ） were as subjects. The peripheral blood samples were taken from every girl. Genomie DNA was amplified by polymerase chain reaction, using primers of 5＂-CAC TGC TGG CTT TTT CAC TGT ATY - 3＂ and 5 ＂-TGA AGG CAG CTG AGA TGG CAA AAA-3 ＂. Then, the PCR product was treated with Msp 1 to examine whether a transition of A to G of nueleotide 1733 of the LHR eDNA existed in these girls. Results The LH level of the girls with early menarehe was higher than the normal ones I P〈0.05 ）, but no statistical difference of FSH level was found between the two groups（ P〉0.05 ）. The Asp57s Gly activating mutation of LHR was not identified in the 177 girls. Conclusion The Asp578 Gly mutation of LHR gene is not related with early menarche.

关 键 词：月经初潮 促黄体激素 基因 突变 学生 

分 类 号：R179[医药卫生—妇幼卫生保健] R339.35[医药卫生—公共卫生与预防医学]