白菜软腐病拮抗菌M-14拮抗机理和拮抗物质的初步研究  

Primary Study on the Antibiotic Mechanism and Substance of M-14 Strain Against Chinese Cabbage Soft Rot

作　　者：唐蕊[1] 张雪辉[1] 石晓云[1] 王僧虎[1] 

机构地区：[1]邢台学院生物化学系,河北邢台054001

出　　处：《北方园艺》2011年第20期38-39,共2页Northern Horticulture

基　　金：河北省科技支撑计划资助项目(10220307)

摘　　要：通过室内筛选得到对白菜软腐病有较强拮抗作用的菌株M-14,对其拮抗机理、拮抗物质的稳定性及抗菌谱进行了初步研究。结果表明:拮抗菌M-14对白菜软腐病菌的拮抗作用源于代谢物质而不是菌体本身;M-14酸碱稳定性极强,在pH 3～11的范围内均表现出较强的抑菌效果;拮抗菌M-14产生的拮抗物质热稳定性较强,在40℃时抑菌活性最强,10～100℃范围内均表现较强的抑菌活性;经对5种供试菌的抑菌作用测试,表现出抗菌谱广的特点。M-14 strain was selected by screening against Chinese cabbage soft rot, the antibiotic mechanism, substance and antimicrobial spectrum were studied. The results showed that the antagonism of M-14 to Chinese cabbage soft rot from metabolites rather than itself bacteria,antibiotic activity was due to the producing substance of M-14 strain, it was very stable in 3- 11 pH values and 10- 100℃ temperatures. In addition,it could inhibit 3 bacteria and 2 fungi, so its restraint spectrum was very wide.

关 键 词：白菜软腐病 拮抗菌 拮抗物质 拮抗机理 

分 类 号：S436.341.13[农业科学—农业昆虫与害虫防治]