丙烯酰胺对大鼠神经元突触可塑性的影响  

Effects of acrylamide on synaptic plasticity of rat neuron

作　　者：肖经纬[1] 孟会林[1] 段化伟[1] 张志荣[1] 王健[1] 鱼涛[1] 郑敏[1] 李斌[1] 郑玉新[1] 

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所,北京100050

出　　处：《中华预防医学杂志》2011年第11期1022-1025,共4页Chinese Journal of Preventive Medicine

基　　金：基金项目：国家自然科学基金（30901219）

摘　　要：目的探讨丙烯酰胺（ACR）对大鼠神经元突触可塑性的影响及机制。方法24只Wistar大鼠按随机数字表完全随机分为对照组（腹腔注射5g/kg生理盐水）和染毒组（腹腔注射30mg／kgACR），每组12只，雌雄各半；采用神经行为学方法评价神经损伤状态，组织病理学法检测心、肝、脾、肺、肾等的超微结构改变，电镜观察脊髓背角突触结构参数的变化，免疫组化法检测大鼠突触特异位点突触素I（SynapsinI）的变化。结果与对照组（雄性：1．00±0．00；雌性：1．00±0．00）比较，染毒组大鼠的步态评分明显增加（雄性：2．50±0．55，t=-7．24，P〈0．01；雌性：3．17±0．41，t=-12．19，P〈0．01）。两组大鼠心、肝、脾、肺、肾等脏器未见病理改变。与对照组[雄性：（0．41±0．09）um；雌性：（0．40±0．06）um]比较，染毒组大鼠的突触活性带长度缩短[雄性：（0．15±0．05）um，t=6．59，P〈0．05；雌性：（0．14±0．07）um，t=7．26，P〈0．05]。对照组和染毒组SynapsinI的定位一致，均位于脊髓背角灰质。对照组SynapsinI呈强阳性反应，而染毒组阳性信号减弱。半定量结果显示，与对照组（雄性：195．40±12．30；雌性：195．19±6．71）比较，染毒组SynapsinI的含量降低（雄性：60．90±29．19，t=10．40，P〈0．05；雌性：67．56±20．23，t=15．65，P〈0．05）。结论ACR神经损伤过程中，大鼠突触结构发生了可塑性改变，并与SynapsinI的表达可能关联。Objective To explore effects of acrylamide on synaptic plasticity of rat neuron and its mechanisms. Methods 24 Wistar rats were divided into control and test groups randomly, 12 rats in each group. The ratio of male and female in each group was 1： 1. Acrylamide （30 mg/kg） was administered to rats by intraperitoneal injection in test group and normal saline（5 g/kg） was given to rats in control group. The neurobehavioral and pathologic changes of heart, liver, spleen, lung and kidney were observed. Changes of parameters in synapse were recorded by electron microscope. As an important target of synapse, change of Synapsin I was measured by immunohistochemical method. Results Compared with the control group （ male： 1.00 ±0. 00; female： 1.00 ±0. 00） ,the gait score was increased significantly in ACR treated group （male： 2.50 ±0.55,t= -7.24,P〈0.01; female： 3.17 ± 0.41,t= - 12. 19,P〈0.01）. No obvious pathological changes of heart, liver, spleen, lung and kidney were found in all rats. Compared with the control group （ male ： （ 0.41 ± 0. 09 ） um; female ： （ 0. 40 ± 0. 06 ） um ）, the length of active zone of synapse was decreased significantly in ACR treated group （ male ： （ 0. 15 ± 0.05 ） um, t = 6. 59, P 〈 0. 05 ; female ： （ 0. 14 ±0. 07 ） um, t = 7. 26, P 〈 0.05 ）. The width and postsynaptic density of synapse in ACR treated group had no significant difference with control group. The location of Synapsin I of control group and ACR treated group was both in gray matter of spinal dorsal horn. Compared with the control group （ male ： 195.40 ± 12. 30; female： 195.19 ± 6.71 ）, the concentration of Synapsin ] was decreased significantly in ACR treated group （ male ： 60.90 ± 29. 19, t = 10. 40, P 〈 0.05 ; female ： 67.56± 20. 23, t = 15.65, P 〈 0. 05 ）. Conclusion Neuronal synaptic plasticity was found in damage of nervous system induced by acrylamide in rats,which might be associated with the exoression of Synapsin I.

关 键 词：丙烯酰胺 突触 神经元可塑性 突触素I 

分 类 号：R114[医药卫生—卫生毒理学]