大豆胚尖再生体系的研究  

Optimization on the Regeneration System of Soybean Embryonic Tips

作　　者：闫帆[1] 孙昕[1] 翟莹[1] 陈虹地[1] 赵健如[1] 李景文[1] 王庆钰[1] 

机构地区：[1]吉林大学植物科学学院,吉林长春130062

出　　处：《大豆科学》2011年第5期757-759,763,共4页Soybean Science

基　　金：转基因生物新品种培育重大专项子课题(2008ZX08004-003);国家自然科学基金面上项目(30971808);吉林省科技发展计划重点项目(20080204);长春市科技局国际科技合作项目(08GH10);"211工程"三期重点学科建设资助项目

摘　　要：以吉大豆1号、2号等5个大豆品种为材料,胚尖为外植体,研究不同浓度6-BA对不同基因型胚尖丛生芽诱芽率和再生率的影响,筛选得到适合于胚尖再生系统的基因型吉大豆2号,并以吉大豆2号为材料,研究侵染阶段加入脯氨酸和硝酸银对胚尖褐化率的影响,将获得的再生植株进行生根试验。结果表明:共培养基中添加3.0 mg.L-1脯氨酸和3.0 mg.L-1硝酸银能显著抑制褐化率,蔗糖配合较高浓度的IBA能更好地促进生根。In order to screen the optimal genotypes for embryonic tip regeneration system in soybean（Glycine max L.Merr.）,the embryonic tips of five soybean cultivars including Jidadou 1 and Jidadou 2,were used as explants to study the effects of 6-benzyl aminopurine（6-BA）concentration on induction and regeneration rate of adventitious bud.The screened soybean cultivar Jidadou 2,fitting for soybean embryonic tip regeneration system,were used as explants to study the influence of Indole-3-butytric acid（IBA）on browning rate of embryonic tips when adding proline and silver nitrate.The browning rate was effectivly inhibited when the concentration of 3.0 mg·L-1 proline and 3.0 mg·L-1silver nitrate were as the additives in co-cultivation medium.Sucrose with a high concentration of IBA could increase the rooting ratio.

关 键 词：大豆 胚尖 基因型 褐化率 生根 

分 类 号：S565.1[农业科学—作物学]