甲基强的松龙对大鼠脊髓损伤后细胞凋亡的影响  

Effect of methylprednisolone on cell apoptosis following spinal cord injury in rats

作　　者：吴继彬[1] 杨惠林[2] 马超[1] 熊传芝 赵猛[1] 王兆红[1] 冯杰[1] 

机构地区：[1]徐州市中心医院骨科,江苏徐州221009 [2]苏州大学附属第一医院骨科,江苏苏州215006

出　　处：《徐州医学院学报》2011年第2期105-108,共4页Acta Academiae Medicinae Xuzhou

基　　金：徐州市科技计划项目（XZZD0916）

摘　　要：目的 观察大剂量甲基强的松龙（methylprednisolone,MP）对大鼠脊髓损伤（spinal cord injury,SCI）后神经细胞凋亡及凋亡相关基因表达的影响.方法 72只健康雌性Wistar大鼠随机分为创伤对照组及MP治疗组,各36只.以Allen′s法制作大鼠SCI模型.MP的给药方法为术后30 min内经鼠尾静脉给予MP 30 mg/kg;创伤对照组给予等容积生理盐水.术后1 h、4 h、12 h、24 h、3 天、7 天处死动物,取出脊髓组织,TUNEL检测细胞凋亡,逆转录-聚合酶链反应（RT-PCR）法检测Bcl-2、Bax mRNA.结果 SCI 24 h时,脊髓组织细胞凋亡达高峰,然后逐渐下降,给予MP治疗后,与创伤对照组比较凋亡指数明显降低（P＜0.05或P＜0.01）.Bcl-2、Bax mRNA结果显示,在SCI 4 h后各时间点MP组大鼠Bcl-2/Bax比值高于创伤对照组,24 h时更明显（P＜0.05或P＜0.01）.结论 细胞凋亡是SCI早期主要的生物学事件;细胞凋亡的调控基因家族Bcl-2/Bax通过调节细胞凋亡参与SCI的发生发展;MP可抑制大鼠SCI细胞的凋亡,从而实现对SCI的影响.Objective To investigate the effect of high dose of methylprednisolone （MP） on neuron apoptosis and the expression of apoptosis - related genes following spinal cord injury （SCI） in rats. Methods 72 healthy female Wistar rats were randomized into trauma control group and MP treatment group （u = 36 each）. Allen＇ s method was used to establish rat SCI model. MP was given via the vena caudalis at a dose of 30 mg/kg within 30 rain after the operation, while the control group was injected with the same dose of normal saline. 1 h, 4 h, 12 h, 24 h, 3 d and 7 d postoperatively, the rats were sacrificed and the spinal cord including the injured site were obtained. The terminal deoxynueleotide transferasemediated DUTP- biotin nick end labeling （TUNEL） was used to observe neural apoptosis; RT- PCR was used to determine the expressions of Bcl - 2 and Bax mRNA in different groups. Results The apoptosis of the spinal tissue reached its peal 24 h after SCI and then began to decline. Following MP treatment, the apoptotic index markedly decreased and there was significant difference compared with the trauma control group （P 〈 0.05 or P 〈 0.01 ）. The resuits of image analysis of Bcl - 2 and Bax mRNA showed that the ratio of Bcl - 2/Bax of MP group higher than that in the trauma control group （ P 〈 0.05 or P 〈 0.01 ）. Conclusion Apoptosis is an important biological event of early SCI ; Bcl -2/Bax, gene family regulating cell apoptosis, participate in the pathogenesis and progression of SCI via tile regulation of the process of apoptosis. MP can inhibit the SCI - induced apoptosis induced in rats.

关 键 词：BAX BCL-2 凋亡 脊髓损伤 

分 类 号：R971[医药卫生—药品] R651[医药卫生—药学]