3个神经组织特异miRNA在不同疼痛中的差异表达及其靶标预测  

Expression of miRNAs and prediction of their targets in diffferent pain models

作　　者：潘志强[1] 郝凌云[1] 杨俊霞[1] 朱丽娇[1] 李燕强[1] 曹君利[1] 

机构地区：[1]徐州医学院江苏省麻醉学重点实验室,江苏徐州221002

出　　处：《徐州医学院学报》2011年第10期643-646,共4页Acta Academiae Medicinae Xuzhou

基　　金：基金项目：国家自然科学基金（81070888,30972834）;东南大学生物电子学国家重点实验室开放课题;徐州医学院院长专项人才基金（09KJZ06）

摘　　要：目的制备慢性炎性疼痛、神经病理疼痛和急性炎性疼痛3种SD大鼠模型，定量检测和分析miR-135b、miR-145和miR-429的表达，结合生物信息学分析预测其作用靶标基因。方法分别采用CFA、CCI和5％甲醛法制备慢性炎性疼痛、神经病理疼痛和急性炎性疼痛动物模型。从大鼠脊髓和背根组织中提取miRNA，并使用反转录方法将其反转为cDNA。使用定量PCR方法对miRNA进行定量分析。使用3个生物信息学预测软件对miR-135b靶标进行分析。结果miR-135b、miR-145和miR-429在不同的疼痛模型中的脊髓和背根组织中均有表达，但表达差异并不一致，其中miR-135b表达差异最显著。miR-135b在慢性炎性疼痛模型的脊髓和背根中的表达分别被上调0．65倍和下调4．3倍，在神经病理疼痛模型的脊髓和背根中的表达分别被上调5．5倍和3倍，而在急性炎性疼痛模型的脊髓和背根的表达中分别被下调1倍和0．6倍。信息学分析表明miR-135b作用的靶标基因为复蛋白基因（Cplxl）、配子生成素结合蛋白2基因（Ggnbp2）、克鲁拜尔基因（Klf4）和多配体蛋白聚糖结合蛋白基因（Sdcbp）。结论miR-135b可能与疼痛发生和维持相关。Objective To analyze the expression of miR - 135b, of miR - 145 and of miR -429 in three different SD rat pain models including CFA pain model, neuro -pathology pain and acute pain model, respectively; and predict the potential targets of the miRNAs with most difference. Methods RT - qPCR was used to detect and analyze the ex- pression of these miRNAs. Three bioformatics softwares including Target, PicTar and RNA22 software were ultilized to predict the miRNA targets. Results Three miRNAs were expressed in the spinal cord and dorsal root ganglion of three different pain models, miR - 145 were all downregulated in SC and DRG of three pain models; the expression of miR - 135b and of miR -429 were not difference in different tissues of different pain models. The analysis of bioinformaties indicated the targets Cplxl, Ggnbp2, Klf4 and Sdcbp for miR - 135b were impossibly closely associated with the methanism of development and maintance of pain. Conclusion miR - 135b may paticipate in the machanism of pain development and maintance.

关 键 词：疼痛 MIRNA 表达 靶标 

分 类 号：Q78[生物学—分子生物学]