阴离子聚电解质诱导硫代黄素T激基缔合物的形成因素分析  

Formation of Thioflavin T Excimer Induced by Anionic Polyelectrolyte and Its Influencing Factors

作　　者：唐琪[1] 孙向英[1] 李芳[1] 

机构地区：[1]华侨大学材料科学与工程学院,福建泉州362021

出　　处：《华侨大学学报（自然科学版）》2011年第6期646-651,共6页Journal of Huaqiao University(Natural Science)

基　　金：国家自然科学基金资助项目(20955001;20575023);福建省自然科学基金资助项目(D0810016);教育部留学回国人员科研启动基金资助项目(2008年度)

摘　　要：研究在水溶液中阴离子聚电解质聚乙烯磺酸钠(PVS)诱导阳离子型染料硫代黄素T(ThT)激基缔合物的形成,考察在PVS存在下,ThT在水溶液中形成激基缔合物的影响因素.通过光谱实验及量化理论计算方法,比较ThT与PVS,PSS和PAA聚电解质之间的相互作用.结果表明:溶液pH值、溶剂和电解质的浓度对ThT激基缔合物的形成均有显著的影响;PSS,PVS可诱导ThT形成激基缔合物,而PAA则无法诱导形成缔合物;通过静电吸引作用,PVS可以增加ThT的局部浓度,从而显著地促进ThT激基缔合物的形成.In this paper, we have studied the formation of thioflavin T （ThT） excimer induced by anionic polyelectrolyte sodium polyethylene sulfonate （PVS）. The influencing factors of the formed Thioflavin T excimer in the presence of PVS were investigated. Furthermore, we have made a study on the interaction of ThT with three kinds of anionic polyeleetrolyte （PVS, PSS and PAA） by spectroscopic techniques as well as computational approach of quantum chemistry computa- tional approach of quantum chemistry. Results showed that pH of solution, concentration of solvent and electrolyte affect the formation of ThT exeimer significantly. PSS and PVS can promote the formation of excimer, but PAA can not PVS can increase partial concentration of ThT by electrostatic attraction process, markedly promoting the formation of excimer,

关 键 词：硫代黄素T 激基缔合物 荧光分析 聚电解质 量子化学 

分 类 号：O631.3[理学—高分子化学] O657.3[理学—化学]