叔丁基异氰酸酯全衍生化β-环糊精SBA-15硅胶手性固定相的研究  

Study on a tert-butylisocyanatedβ-Cyclodextrin bonded SBA-15 chiral stationary phase for fast enantioseparation

作　　者：李来生[1] 马海萍[1] 方奕珊[1] 陈红[1] 

机构地区：[1]南昌大学分析测试中心,南昌330047

出　　处：《分析试验室》2011年第11期20-25,共6页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(21165012);江西省自然科学基金(2010GZH0089);江西省教育厅科技项目(GJJ11274)资助

摘　　要：合成和表征了一种叔丁基异氰酸酯全衍生化β-环糊精SBA-15硅胶手性固定相。在RP-HPLC条件下,采用5-cm短柱,分别实现了对包含β-受体阻滞剂普萘洛尔(propranolol)在内的8种含氮原子手性药物对映体的拆分,初步考察了流动相的组成、pH及流速对手性色谱分离的影响。上述药物对映体均在短时间(t<6 min)内得到了良好的分离。a novel chiral stationary phase was successfully prepared by immobilization of （mono-6-ethylene diamino-6-deoxy-2,3,6-tert-butylisoeyanated） β-eyclodextrin onto the surface of a mesoporous silica gel （SBA- 15）. The resulting bonded silica stationary phase was characterized by instrumental analyses. Eight nitrogencontaining chiral enantiomers including four β-blocker drugs and others were successfully separated in RPHPLC, respectively. The effects of composition, buffer concentration and pH value in mobile phases on the chiral selectivity of β-blockers were also preliminarily investigated. The results showed that the short CD-based 5era-column exhibited rapid separation ability to the above drugs within 6 min, which indicates that the separation has great potential in fast enantioseparation. The established method is simple, fast, efficient and reproducible for the above chiral drugs.

关 键 词：液相色谱法 环糊精类手性固定相 SBA-15硅胶 手性药物 手性分离 

分 类 号：O652.6[理学—分析化学]