微流控二维芯片电泳技术在蛋白质分离分析中的应用  

Developments of Electrophoresis Separation and Analysis of Proteins on the Two-dimensional Microfluidic Chip

作　　者：甘俊[1,2] 徐溢[1,2,3] 倪雅楠[1,2] 马亮波[1,2] 彭金兰[1,2] 王尧[1] 

机构地区：[1]重庆大学化学化工学院,重庆400030 [2]新型微纳器件与系统技术国防重点学科实验室,重庆400030 [3]微纳系统及新材料技术国际研发中心,重庆400030

出　　处：《功能材料与器件学报》2011年第5期491-499,共9页Journal of Functional Materials and Devices

基　　金：国家自然科学基金【20675089】;全国博士学位论文作者专项资金资助项目【200941】;国家科技部"创新药物孵化基地"子课题项目【2010ZX09401-306-1-5】资助;重庆大学研究生科技创新基金项目【CDJXS10-22-11-39】;中央高校基本科研业务费【CDJXS1-22-00-05】资助

摘　　要：微流控二维芯片电泳技术因其具有分离速度快、易于微型化和自动化、接口处死体积小、可极大地改善峰容量和分辨率等优点,而在复杂的蛋白质组学研究中展现出了巨大的潜力。文中针对蛋白质微流控二维芯片电泳分离原理、芯片的设计和制作、检测技术、分离分析条件的优化等进行了综述,按二维接口模式分类讨论了微流控二维芯片电泳技术在蛋白质分离分析中的研究现状和最新进展,并就微流控二维芯片技术在蛋白质组学研究中的应用前景进行了展望。Two - dimensional microfluidic chip electrophoresis technology has shown great potential in the study of complex proteomics because of its advantages, such as fast separation speed, easy miniaturization and automation, lower dead volume at the interface, high peak capacity and resolution, and so on. In this paper, the principles of two-dimensional protein microfluidic chip electrophoresis, the chip design and fabrication, detecting method, optimizing conditions of separation and analysis were summarized . According to two - dimensional interface modes, the research and the latest developments of the two - dimensional microfluidic chip electrophoresis in protein separation and analysis were reviewed. At last, the application prospects of two - dimensional microfluidic chip electrophoresis in the proteomics research was also discussed.

关 键 词：微流控芯片 二维芯片电泳 蛋白质 分离 检测 

分 类 号：O652[理学—分析化学] Q51[理学—化学]