贝氏高原鳅不同组织原代培养的研究  

Primary Culture of Different Tissues from Triplophysa bleekeri(Sauvage et Dabry)

作　　者：黄静[1] 王志坚[1] 霍静 

机构地区：[1]淡水鱼类资源与生殖发育教育部重点实验室,水产科学重庆市市级重点实验室,西南大学生命科学学院,重庆400715

出　　处：《西南师范大学学报（自然科学版）》2011年第5期145-150,共6页Journal of Southwest China Normal University(Natural Science Edition)

摘　　要：采用胰酶半消化法,对贝氏高原鳅的吻端、肾脏和性腺组织的原代培养条件进行探究,使用MEM,RPMI1640,DMEM和DMEM/F12培养基进行培养;血清质量分数设置为10%,30%,50%和100%;分别置于37℃,26℃和室温(11～15℃)条件下培养,观察细胞在不同条件下的生长情况.初步确定了各组织原代培养的最佳条件是温度26℃、血清质量分数50%的条件下,吻端和肾脏组织在MEM培养基、精巢在DMEM培养基中生长情况最优.Giemsa染色显示各组织细胞结构完整,荧光染料Alexa fluor 488Phalloidin显示各组织细胞的微丝网络发达.By using the semidigestive tissue explantation technique,primary culture of cells derived from the snout,kidney,spermary tissues of Triplophysa bleekeri,were conducted.Those tissues were cultured in MEM medium,RPMI 1640 medium,DMEM medium and DMEM/F12 medium;10%,20%,30% and 100% fetal bovine serum and incubated at room temperature（11-15 ℃）,temperature 26 ℃ and 37 ℃.The growth of different tissues in different conditions were compared.The optimized conditions for primary culture of spermary tissue are：DMEM medium,26℃ 50% fetal bovine serum and that for primary culture of kidney and snout tissues are：MEM medium,26℃ 50% fetal bovine serum.Giemsa stain shows the structure of those tissue cells is integrity.Fluorescent dye Alexa fluor 488 Phalloidin shows the microfilament networks of those tissue cells are well-developed.

关 键 词：贝氏高原鳅 组织 原代培养 条件优化 微丝 

分 类 号：Q959.483[生物学—动物学]