HPLC法测定不同采收期丹参中丹参酮ⅡA及丹酚酸B含量  

Determination of TanshinoneⅡA and Salvianolic Acid B in Radix Salvia Miltiorrhiza from different harvest time by HPLC

作　　者：张珺[1] 蓝建芳 关亚丽[1] 刘颖硕[1] 程月发[1] 

机构地区：[1]河北联合大学冀唐学院,河北唐山063000 [2]海南赛立克药业有限公司,海南海口571124

出　　处：《齐鲁药事》2011年第10期572-573,共2页qilu pharmaceutical affairs

摘　　要：目的测定同一产地不同采收期丹参药材中丹参酮ⅡA及丹酚酸B的含量。方法以丹参酮ⅡA和丹酚酸B为定量评价指标,高效液相色谱法进行含量测定。结果 6批次丹参药材中丹参酮ⅡA质量分数为0.23%～0.36%,平均为0.29%;10批次丹参中丹酚酸B的质量分数为3.59%～4.48%,平均为3.97%。结论相同产地不同采收期丹参药材中丹参酮ⅡA及丹酚酸B的含量存在一定差异。Objective To compare the content of tanshinone II A and salvianolic acid B in Radix Salvia Mihiorrhiza from different harvest time planted in a same growing area. Methods The contents of tanshinone IIA and salvianolic acid B in Radix Salvia Mihiorrhiza were determined by HPLC on a C18 column,and the flow rate was 1 mL·min-1. The column temperature was 25 ℃ and the quantification used external standard method. The contents of tanshinone IIA were determined with methanol - water（75： 251 as mobile phase ,and the wavelength of UV detector was 270 nm. The contents of salvianolic acid B were determined with methanol -acetonitrile -formic acid -water （30： 10： 1：59） as mobile phase, and the wavelength of UV detector was 286 nm. Results The contents of tanshinone IIA were ranged from 0.23% - 0.36% and the average was 0.29%. The contents of salvianolic acid B were ranged from 3.59% - 4.48% and the average was 3.97%. Conclusion There was relatively large difference in the contents of tanshinone IIA and salvianolic acid B from different harvest time which were cultivated in a same growing area.

关 键 词：丹参 丹参酮ⅡA 丹酚酸B 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]