阿维链霉菌的诱变育种  

The Mutation Breeding of Streptomyces avermilitis

作　　者：陈金辉[1] 郭伟群[1] 蔡玉娟[1] 王天科[1] 王晓芳[1] 宋渊[1] 

机构地区：[1]中国农业大学生物学院,北京100193

出　　处：《生物技术进展》2011年第2期135-139,共5页Current Biotechnology

基　　金：国家科技支撑计划项目(2006BAD08B02)资助

摘　　要：以阿维链霉菌(Streptomyces avermitilis)76-12为出发菌株,采用亚硝基胍、吖啶橙、紫外线和氯化锂分别对其孢子和原生质体进行诱变,经抗代谢物理性筛选,获得一系列高产突变株,其中N-1-2高产突变株的发酵单位是出发菌株的2.47倍。实验中同时获得了只产阿维菌素a组分的突变株G-32、Bla组分含量高的Ave8菌株和产蓝绿色孢子的突变株UA-G等。Spores and protoplasts of the original strain Streptomyces avermitilis 76 12 were treated with nitrosoguanidine （NTG）, acridine orange, ultraviolet radiation （UV） and LiCl respectively. Then a series of high yield avermectin producing and stable mutants were obtained through mutation and rational selection. The avermection productivity of the mutant N 1 2 was 2.47 times of the original strain. Furthermore, several special mutants were obtained. Mutant strain G 32 produced only ＂a＂ avermectins; mutant strain Ave8 had higher lever of avermectin B1a content than original strain; and mutant strain UA G developed bluegreen spores.

关 键 词：阿维菌素 阿维链霉菌 诱变 理性筛选 

分 类 号：Q933[生物学—微生物学]