鞘内注射艾司洛尔对福尔马林疼痛大鼠脊髓背角c-fos蛋白表达的影响  

Effects of intrathecal esmolol on spinal c-fos expression in a formalin-induced pain model

作　　者：陈烨[1] 邹聪华[1] 陈彦青[1] 

机构地区：[1]福建医科大学省立临床学院(福建省立医院)麻醉科,福州市350000

出　　处：《临床麻醉学杂志》2011年第10期1014-1016,共3页Journal of Clinical Anesthesiology

摘　　要：目的探索大鼠鞘内注射艾司洛尔能否抑制福尔马林大鼠脊髓背角c-fos蛋白表达。方法雄性SD大鼠，鞘内置管成功后，随机分为三组（n=8）：生理盐水组（N组），艾司洛尔300μg／kg组（E1），艾司洛尔600μg／kg组（E2组）。在异氟醚麻醉下，鞘内注射上述药物10μl，5d后，所有大鼠一侧后肢爪掌背侧皮下注射福尔马林（5％，50μl），观察大鼠在Ⅰ相（0～5min）和Ⅱ相（15-50min）疼痛行为学变化（包括添足、咬足、抬腿动作等），并进行Dubuisson评分。采用免疫组织化学方法检测脊髓背角c—fos蛋白表达。结果与N组比较，E1、E2组大鼠Ⅱ相阶段的疼痛相关行为均有减少，呈量效关系（P〈0．05），且E1、E2组L4～5节段脊髓表达的c-fos蛋白均较N组减少（P〈0．05）。结论鞘内注射艾司洛尔可减少福尔马林大鼠脊髓背角c—fos蛋白表达。Objective To investigate the effect of intrathecal esmolol on the expression of c-fos in the dorsal horn of spinal cord in a formalin induced pain model Methods Twenty-four SD rats were randomly divided into 4 groups with 8 each. Every mouse received intrathecal catheter placement and was infused with saline 10 L（group N）, esmolol 300 g/kg（group A）, or 600 g/kg（group B）, respectively. Pain was induced by 50 L of 5 % formalin solution injected into the dorsal surface of the right hind paw. The noeiceptive behaviors including licking, biting and lifting of the injected paw were cumulatively recorded during phase Ⅰ （0 -5 min） and phase Ⅱ （15 -50 min） by Dubuisson＇s method. The expression of c-fos protein in the spinal dorsal horn was detected with immunohistochemistry. Results Compared with group N, the pain-related behaviors in phase Ⅱ were decreased significantly in group A and B. The expression of c-fos in the L4- 5 spinal dorsal horn was significantly lower in group A and B compared with group N（P〈0.01）. Conclusion Intrathecal morphine can inhibit c-fos expression in the spinal dorsal horn in a formalin-induced pain model.

关 键 词：艾司洛尔 福尔马林 疼痛 脊髓C-FOS 

分 类 号：R614[医药卫生—麻醉学]