野蚕孵化酶基因的克隆与生物信息学分析  

Cloning and Bioinformatic Analysis of Hatching Enzyme Gene in Chinese Wild Silkworm(Bombyx mandarina)

作　　者：唐顺明[1,2] 赵新慧[1] 吴俊[1] 裘智勇[1,2] 沈兴家[1,2] 

机构地区：[1]江苏科技大学,江苏镇江212003 [2]中国农业科学院蚕业研究所农业部蚕桑遗传改良重点开放实验室,江苏镇江212018

出　　处：《江西农业学报》2011年第10期1-5,共5页Acta Agriculturae Jiangxi

基　　金：国家高技术研究发展计划863重点项目(2007AA100504);江苏省自然基金项目(SBK200930217);江苏省高校自然科学基金项目(08KJB230001)

摘　　要：采用RT-PCR法从野蚕胚胎中克隆了一个885 bp孵化酶基因,命名为BmandHE(GenBank登录号:JN620366)。BmandHE ORF编码294个氨基酸残基,蛋白分子质量为33.57 kD,等电点为5.55。在BmandHE N-端存在16个氨基酸的信号肽,BmandHE序列含有锌指结合序列、Met-转角基序等孵化酶保守功能区域和构成孵化酶二级结构骨架的4个半胱氨酸残基。BmandHE与其他动物孵化酶氨基酸序列的相似性为30.4%～97.1%。进化分析表明,野蚕与昆虫家蚕、柞蚕、家蚊聚为一类,亲缘关系比较近。By means of RT-PCR technology,we cloned a 885 bp（in length） hatching enzyme（HE） gene from the embryo of Chinese wild silkworm（Bombyx mandarina）,and this gene was designated as BmandHE（GenBank accession： JN620366）.BmandHE ORF encodes 294 amino acids residues with the predicted molecular weight of 33.57 kD and the isoelectric point of 5.55.The deduced BmandHE sequence had a signal peptide of 16 amino acids in N terminator.The BmandHE sequence contained zinc-binding domain and Met-turn domain,which are the characteristic domains of HE.Moreover,it had four cysteine amino acid residues in the fixed positions,which are the backbone for the second structure of HE.Homologous analysis results of the deduced protease domain showed that BmandHE had identity of 30.4% ~97.1% to the HE in other species.Evolution analysis revealed that Bombyx mandarina had close relation with Bombyx mori,Antheraea pernyi and Culex quinquefasciatus,and they had close genetic relationship.

关 键 词：野蚕 孵化酶基因 ORF 克隆 生物信息学分析 

分 类 号：S888.72[农业科学—特种经济动物饲养]