基于纳米金标记的适体传感器的研制  

Research and fabrication of aptamer sensor based on nano-gold labeled

作　　者：常艳兵[1,2] 李晓飞[1] 张银烽[1] 司剑飞[1] 李月婷[1] 杨云慧[1] 

机构地区：[1]云南师范大学化学化工学院,云南昆明650092 [2]曲靖师范学院继续教育学院,云南曲靖655000

出　　处：《传感器与微系统》2011年第11期108-110,114,共4页Transducer and Microsystem Technologies

基　　金：国家自然科学基金资助项目(20865006)

摘　　要：采用乙醇热溶剂法合成了TiO2纳米带,将所合成的TiO2纳米带与可卡因适体和壳聚糖混合后修饰到玻碳电极上,研制了一种新型的适体传感器用于可卡因的检测。考察了适体传感器的电化学特性,同时研究了适体传感器进行检测电解液的pH值、培育时间和反应时间等实验条件对传感器性能的影响。该传感器在可卡因浓度为1×10-6～1.5×10-4 mol/L的范围内有良好的线性关系,检测下限为4.0×10-7mol/L。实验结果表明:该适体传感器操作简便、灵敏度高、选择性好,具有良好的重现性和稳定性。A new type of aptamer sensor for rapid detection of cocaine is developed. TiO2 nanobelts are synthesized using ethanol hot solvent method. TiO2 nanobelts and cocaine aptamer are immobilized onto the surface of glassy carbon electrode. The electrochemical characteristics of aptamer sensors are investigated. The effect of pH of electrolyte, incubation time, reaction time and other experimental conditions on the characteristic of the sensor are also studied. The prepared aptamer sensor offered an amperometric response for cacoine ranging from 1 ×10^-6- 1.5 ×10^-4 mol/L with a detection limit of 4. 0×10^-7 mol/L. Experimental results show that the aptamer sensor has advantages of easy to operate, high sensitivity, good selectivity, good reproducibility and stability.

关 键 词：热溶剂法 TIO2纳米带 可卡因 金标DNA 适体传感器 

分 类 号：O657[理学—分析化学]