祈州漏芦的组织培养与快繁技术研究  

Tissue Culture and Rapid Propagation of Rhaponticum uniflorum

作　　者：赵春艳[1] 刘金成[1] 方坤[1] 王宏波[2] 

机构地区：[1]哈尔滨医科大学大庆校区,黑龙江大庆163319 [2]哈尔滨医科大学附属五院,黑龙江大庆163319

出　　处：《中国食物与营养》2011年第10期28-29,共2页Food and Nutrition in China

基　　金：黑龙江省教育厅科学技术研究项目"大庆地区祁州漏芦资源的开发利用"(项目编号:2008-11531183)

摘　　要：目的:对祁州漏芦愈伤组织诱导培养基和生长的培养条件进行研究,构建祁州漏芦快速繁殖体系。方法:通过筛选外植体,添加不同种类及浓度的生长调节剂对祁州漏芦进行愈伤组织诱导、芽增殖培养及生根培养。结果:诱导愈伤组织适宜培养基:MS+0.3mg/L NAA+3.0mg/L 6-BA;芽增殖培养以MS+0.3mg/L NAA+5.0mg/L 6-BA为宜;生根培养以1/2MS+0.1mg/L NAA为佳。结论:利用组织培养技术能够实现祁州漏芦快速繁殖。【Objective】 To provide some new evidences for rapid propagation of Rhaponticum？ uniflorum through studying on medium with callus tissue culture and the condition of growth.【Method】 Callus tissue induction,bug proliferation culture,rooting culture of Rhaponticum uniflorum were conducted by explants selecting and growth regulator adding different hormones.【Result】 The appropriate medium for different culture stages were MS＋0.3mg/L NAA＋3.0mg/L 6-BA in callus culture induction,MS＋0.3mg/L NAA＋5.0mg/L 6-BA in differentiation and subculture of cluster buds,1/2MS＋0.1mg/L NAA in rooting culture.【Conclusion】Rapid propagation can be achieved with plant tissue culture in Rhaponticum uniflorum.

关 键 词：祁州漏芦 组织培养 快速繁殖 

分 类 号：S567.239[农业科学—中草药栽培]