替代对照品法测定龙血竭原料中龙血素A和B的含量  

Determination of loureirin A and loureirin B in Dracaena cochinchinensis by substitute reference substance

作　　者：许佳 金红宇[2] 孙磊[2] 

机构地区：[1]北京市朝阳区药品检验所,北京100028 [2]中国食品药品检定研究院,北京100050

出　　处：《药物分析杂志》2011年第11期2058-2062,共5页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立替代对照品法同时测定龙血竭原料剑叶龙血树中龙血素A、B的含量。方法:提出了相对斜率的概念,解释了它与相对校正因子的数学关系,阐释了相对斜率(相对校正因子)的物理意义,进而采用相对斜率算法建立剑叶龙血树的含量测定法,并进行方法学验证。结果:方法的线性和回收率均良好;以龙血素A为参照,龙血素B的相对斜率和相对保留时间平均值为1.248和1.06,且在5个不同的色谱系统上保持稳定;相对斜率值与使用紫外分光光度计按吸光系数法测得值一致。结论:相对斜率算法简便、准确,以此进行替代对照品法测定剑叶龙血树中龙血素A、B是可行的。Objective： To establish an HPLC method for determination of loureirin A and loureirin B in Dracaena cochinchinensis by substitute reference substance. Method： To give the definition and physical interpretation of relative slope （RS） , explain the relationship between RS and relative correction factor. The determination of loureirin A and loureirin B was established and validated. Results： The quantitative analysis was demonstrated with good linear relationship and recoveries. The RS and relative retention time of loureirin B detected by five different HPLC systems showed good repeatabilities. And mean values were 1. 248 and 1.06. The values of RS detected by HPLC and ultraviolet spectrophotometry were equal. Conclusion： The method is simple and accurate, can be used for determination of loureirin A and loureirin B in D. cochinchinensis.

关 键 词：民族药 龙血竭 剑叶龙血树 龙血素A 龙血素B 高效液相色谱法 替代对照品法 一测多评 相对斜率 

分 类 号：R917[医药卫生—药物分析学]