高速逆流色谱分离纯化紫苏叶中迷迭香酸  

High-speed counter-current chromatography separation and purification of rosmarinic acid from Perilla frutescens(L.) Britt.var.acuta(Thunb.) Kudo form.discolor(Makino) Kudo

作　　者：黄亮辉[1] 赵英永[1] 王李丽[1] 程显龙[2] 林瑞超[2] 孙文基[1] 

机构地区：[1]西北大学陕西省生物医药重点实验室,西安710069 [2]中国食品药品检定研究院,北京100050

出　　处：《药物分析杂志》2011年第11期2087-2090,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金(No.81001622)

摘　　要：目的:建立高速逆流色谱分离纯化紫苏叶中迷迭香酸的方法。方法:采用高速逆流色谱分离纯化紫苏叶乙酸乙酯萃取部分中迷迭香酸,以石油醚-乙酸乙酯-甲醇-0.5%醋酸水溶液(2∶5∶2∶5)为溶剂体系,上相为固定相,下相为流动相,流速2.0 mL.min-1,主机转速800 r.min-1,温度25℃。结果:每0.20 g紫苏叶乙酸乙酯萃取物经1次纯化,纯度为98.6%的迷迭香酸的得率约13%。结论:高速逆流色谱可高效分离纯化紫苏叶中的迷迭香酸。Objective： To establish a method for separation and purification of rosmarinic acid from Perilla frutescens（ L. ） Britt. vat. acuta （ Thunb. ） Kudo form. discolor（ Makino ） Kudo （ abbr： PFAD） by high - speed counter - current chromatography（ HSCCC ）. Methods： The compound was separated and purified by HSCCC from the ethyl acetate part of the crude extract of PFAD, using petroleum ether - ethyl acetate - methanol - 0. 5 % acetic acid water -solution（2： 5： 2：5 ）as the two -phase solvent system. The upper phase was chosen as the stationary phase, and the lower phase was chosen as the mobile phase. The mobile phase was operated at a flow rate of 2. 0 mL ·min^-1, while the apparatus rotated at 800 r ·min^-1 and the temperature was set at 25 ℃. Results： The yield of rosmarinic acid with 98.6% purity was 13% from each 0.20 g ethyl acetate part of the crude extract of PFAD by once separation. Conclusion： HSCCC is a efficient method for separating, and purificating rosmarinic acid from PFAD.

关 键 词：紫苏叶 迷迭香酸 高速逆流色谱 分离 标准物质 电喷雾-质谱 鉴定 

分 类 号：R917[医药卫生—药物分析学]