黄参多糖对癌细胞HepG2和P_(388)增殖抑制与细胞凋亡的影响  

Effect of Sphallerocarpus gracilis Polysaccharides on Proliferation Inhibition and Apoptosis of Sarcoma HepG2 and Mouse Lymphocyte Leukemia P_(388)

作　　者：贾磊[1] 聂秀娟[1] 方梅[2] 

机构地区：[1]黄山学院体育系,安徽黄山245041 [2]甘肃农业大学动物医学院,甘肃兰州730070

出　　处：《食品科学》2011年第19期227-231,共5页Food Science

基　　金：安徽省教育厅自然科学项目(KJ2009B274Z);黄山学院引进人才启动项目(2008xskq013)

摘　　要：目的:探索黄参提取物黄参多糖(SGP)对肝癌细胞HepG2和淋巴白血病细胞P388增殖的抑制与诱导细胞凋亡的效应,并初步探讨其对生命延长的作用。方法:应用SGP与肝癌细胞HepG2和淋巴白血病细胞P388共培养,采用MTT比色法测定不同剂量SGP对HepG2和P388细胞增殖的抑制;通过对小鼠皮下移植性肿瘤HepG2生长抑制实验,用流式细胞术分析SGP诱导细胞凋亡和细胞周期分布的变化;探讨SGP对荷淋巴白血病小鼠生命延长的作用。结果:SGP可明显抑制体外培养HepG2和P388细胞的增殖,200mg/(kg.d)剂量作用48h,对HepG2的抑制率可达37.05%(P<0.01),而150、200mg/(kg.d)剂量作用P388细胞48h后抑制率均超过38%(P<0.01);SGP在小鼠体内也能明显抑制HepG2移植性肿瘤生长,其抑制率超过40%;可诱导移植性肝癌HepG2细胞凋亡,并使细胞时相分布在G1期发生阻滞;对荷P388(腹水)小鼠生命有延长作用,延长率达10%。结论:SGP对小鼠有良好的抗癌变效果以及对小鼠原发性肝癌和淋巴白血病的良好抑制作用。Objective： To explore the effect of Sphallerocarpus gracile polysaccharides（SGP） on apoptosis induction and proliferation inhibition of HepG2 and P388 cells as well as the lifespan extension of mice treated with SGP.Methods： HepG2 and P388 cells were co-cultured in the presence of SGP in this study.The inhibitory effect of SGP at different concentrations on the proliferation of HepG2 and P388 was evaluated by MTT assays.The SGP-induced apoptosis and cell cycle change of HepG2 and P388 were analyzed by flow cytometry.Meanwhile,the extension of lifespan of P388 mice treated with SGP was determined.Results： SGP revealed a significant anti-proliferation effect on HepG2 and P388 in vitro.The inhibition rate of SGP treatment for 48 h at the concentration of 200 mg/（kg·d） against HepG2 was 37.5%（P0.01）.The inhibition rates against P388 at 150 and 200 mg/（kg·d） after 48 h of treatment were both over 38%（P0.01）.In addition,SGP obviously inhibited the growth of implanted HepG2 in mice with an inhibitory rate of higher than 40%.Moreover,SGP induced the apoptosis of HepG2 and block HepG2 at the G1 phase.Furthermore,SGP prolonged the life of P388-bearing mice with a lifespan extension rate of 10%.Conclusion： SGP has an outstanding anti-cancer effect in mice and can effectively inhibit the proliferation of HepG2 and P388 cells and induce the apoptosis of HepG2 cells.

关 键 词：黄参多糖 HEPG2细胞 P388细胞 增殖抑制 细胞凋亡 

分 类 号：R734.2[医药卫生—肿瘤]