无机与有机碳源混合营养培养对钝顶螺旋藻生长的影响  

The Influence of Inorganic and Organic Carbon Sources on Mixtrophic Growth of Spirulina platensis

作　　者：田辉[1,2] 谯顺彬[3] 彭晓东[1,2] 李林[1] 张义明[2,3] 

机构地区：[1]贵州大学化学与化工学院,贵州贵阳550003 [2]贵州省发酵工程与生物制药重点实验室,贵州贵阳550003 [3]贵州工业职业技术学院,贵州贵阳550008

出　　处：《贵州农业科学》2011年第10期122-124,共3页Guizhou Agricultural Sciences

基　　金：贵州省自然科学基金项目"混合营养培养对螺旋藻光合色素含量的影响的研究"[黔科合J字(2010)2068];贵州省优秀科技教育人才省长资金项目"螺旋藻在航天领域构建循环生态环境作用的模拟研究";贵州大学研究生创新基金项目"混合营养培养对钝顶螺旋藻及藻胆蛋白含量影响的研究"[校研理工2011052]

摘　　要：为深入考察碳源混合营养对螺旋藻生长的影响,采用响应面分析法对螺旋藻混合营养培养中的无机碳源(NaHCO3)与有机碳源(葡萄糖)进行2因素3水平试验。结果表明:单因素试验螺旋藻在基础培养基条件下,添加1.5g/L葡萄糖时,最终生物量为1.242 9g/L;在以最佳葡萄糖添加浓度时,NaHCO3的最佳添加浓度为8.4g/L;经响应面分析,螺旋藻混合营养培养时,无机碳源与有机碳源两者之间存在交互作用。In order to further examine the carbon impact on mixtrophic growth of S.platensis.A test of two factors and three levels was conducted to study the effects of NaHCO3 and glucose on mixtrophic growth of S.platensis by response surface analysis method.The single factor experiment results showed that under the condition of S.platensis in basic medium,the final biomass could be up to 1.242 9 g/L by adding 1.5 g/L glucose.The optimum adding concentration of NaHCO3 was 8.4 g/L according to that of glucose.The response surface analysis showed that there were interactions between inorganic and organic carbon sources in mixtrophic growth of S.platensis.

关 键 词：钝顶螺旋藻 混合营养培养 有机碳源 无机碳源 

分 类 号：S968.4[农业科学—水产养殖]