TLR激动剂对中性粒细胞Bax蛋白表达的影响  

EFFECT OF TLR LIGANDS ON INTRACELLULAR BAX EXPRESSION OF NEUTROPHILS

作　　者：高燕[1] 叶迎春[1] 王栩[1] 黄黎[1] 曹新梅[1] 刘佳佳[1] 

机构地区：[1]四川省泸州医学院免疫学教研室,泸州646000

出　　处：《现代预防医学》2011年第21期4451-4453,共3页Modern Preventive Medicine

基　　金：国家自然科学基金资助(30371305)

摘　　要：[目的]研究TLR激动剂LPS和PGN影响中性粒细胞凋亡及凋亡相关的Bax蛋白的表达。[方法]分离培养正常受试者的外周血中性粒细胞,分别加入LPS和PGN两种TLR激动剂,并设生理盐水对照,用流式细胞术检测中性粒细胞的凋亡抑制率,通过Westernbloting技术检测中性粒细胞Bax蛋白的表达。[结果]LPS和PGN降低中性粒细胞自发性凋亡的发生率,与自发性凋亡组比较差异有统计学意义(P﹤0.05);接受LPS和PGN刺激的中性粒细胞表达Bax蛋白均低于自发性凋亡组(P﹤0.05)。[结论]在TLR激动剂诱发的中性粒细胞生存效应中,LPS和PGN可能通过降低中性粒细胞内Bax蛋白的表达来延长中性粒细胞的生命期限。[Objective]In order to explore new progress about neutrophil survival,and the effects of TLR ligands on apoptosis and intracellular Bax protein expression.[Methods]Heparinized peripheral blood was obtained from healthy volunteers.The neutrophils were cultured at the presence or absence of TLR ligands（LPS and PGN）for the indicated time periods.Apoptosis of neutrophils was detected by flow cytometric analysis.The expression of Bax protein of neutrophils was examined by Western blotting analysis.[Results]Flow cytometry indicated that LPS and PGN delayed neutrophil apoptosis.Western blotting showed that the expression of Bax protein in nuetrophils was down-regulated by the TLR ligands.[Conclusion]LPS and PGN might delay spontaneous apoptosis of neutrophils by reducing the expression of Bax protein in neutrophils.

关 键 词：中性粒细胞 凋亡 TLR BAX 

分 类 号：R392.12[医药卫生—免疫学]