水体中铜对中华鲟幼鱼血液生化指标的影响  

Effects of dissolved copper in water body on serum biochemical indices of juvenile Chinese sturgeon(Acipenser sinensis)

作　　者：章龙珍[1,2] 姚志峰[1,2] 庄平[1] 黄晓荣[1] 赵峰[1] 王妤[1,3] 

机构地区：[1]中国水产科学研究院东海水产研究所,农业部海洋与河口渔业资源及生态重点开放实验室,上海200090 [2]上海海洋大学水产与生命学院,上海201306 [3]大连海洋大学生命科学与技术学院,辽宁大连116023

出　　处：《生态学杂志》2011年第11期2516-2522,共7页Chinese Journal of Ecology

基　　金：国家自然科学基金重大项目(30490234);上海市科委科研计划项目(08391910300);农业部公益性行业科研专项(200903048-07);上海市长江口中华鲟保护区专项(沪鲟保科-20091212003);中央级公益性科研院所基本科研业务费专项资金项目(2008M01)资助

摘　　要：通过检测中华鲟在不同铜离子浓度的水体中暴露60d后血液生化指标,研究Cu2+对幼鱼血浆生化成分和离子含量变化的影响及致毒效应。结果表明:中华鲟幼鱼血浆中血糖(Glu)、碱性磷酸酶(ALP)、尿素(Urea)、胆固醇(TC)、肌酐(CREA)含量随Cu2+浓度的增加而升高,低浓度组(0.40μg.L-1)ALP含量与对照组有显著性差异(P<0.05),Glu、Urea、TC、CREA含量无显著性差异;中浓度组(0.89μg.L-1)和高浓度组(2.00μg.L-1)含量与对照组均有显著性差异(P<0.05)。甘油三酯(TG)随着Cu2+浓度的增加而下降,低浓度组与对照组无显著性差异;中、高浓度组与对照组有显著差异(P<0.05),总蛋白(TP)、谷草转氨酶(AST)、谷丙转氨酶(ALT)、乳酸脱氢酶(LDH-L)不受Cu2+的影响。Na+、Cl-、P含量和pH值随Cu2+浓度的增加而显著下降,Ca2+、Mg2+显著上升,其变化与Cu2+浓度存在相关性,K+含量不受Cu2+的影响。实验表明,Cu2+对中华鲟幼鱼血液生化指标的最低可观察效应浓度和最高无观察效应浓度分别为0.89和0.4μg.L-1。血浆中ALP受Cu2+影响最明显,其含量除受Cu2+浓度影响外,随着时间的延长也显著升高,是Cu2+污染的敏感指标。Detections were made on the serum biochemical indices and ion concentrations of juvenile Chinese sturgeon （Acipenser sinensis） after exposed to the water body added with different concentration Cu2＋ for 30 and 60 days, aimed to understand the toxic effect of Cu2＋ on the juveniles. With the increasing concentration of Cu2＋ in water body, the concentrations of glucose （Glu）, alkaline phosphatase （ALP）, urea, total cholesterol （TC）, and creatinine （CREA） in the serum increased, while that of triglycerides （TG） decreased. The Cu2~ had little effects on the concentrations of total protein （TP）, aspartate aminotransferase （AST）, alanine aminotrans- ferase （ALT）, and lactate dehydrogenase （LDH）, and there were no significant differences in the test biochemical indices except ALP between the treatment low concentration Cu2＋ and the control. At medium （0.89 μg . L-1 ） and high （2.00 μg .L-1 ） concentrations of Cu2＋, the concentrations of GLU, ALP, urea, TC, CREA, and TG were significantly higher than those of the control （ P〈0.05 ）. The pH and Na＋, CI-, and P concentrations decreased significantly with increasing concentration of Cu2＋ while the Ca2＋ and Mg2＋ concentrations were in adverse, and the K＋ concentration was less affected. The minimum observable and the maximum unobservable effect concentrations of Cu2＋ were 0.89 and 0.40 μg . L-1 , respectively. Among the test indices, ALP was most affected by Cu2＋, and increased significantly with increasing time of Cu2＋ exposure, being able to be the sensitive index of juvenile Chinese sturgeon under water body Cu2＋ pollution.

关 键 词：中华鲟幼鱼 铜 血浆 生化指标 离子 

分 类 号：S917.4[农业科学—水产科学]