莱氏野村菌不同菌株生物学特性及其对甜菜夜蛾的毒力  

Biological Characters of Nomuraea rileyi Strains and Their Virulences against Spodoptera exigua(Hübner)(Lepidoptera:Noctuidae)

作　　者：杜广祖[1] 陈斌[1] 李正跃[1] 肖肖[1] 

机构地区：[1]生物多样性控制作物病虫害教育部重点实验室,生物多样性控制病虫害应用技术国家工程研究中心,云南农业大学植物保护学院,云南昆明650201

出　　处：《西南农业学报》2011年第5期1761-1765,共5页Southwest China Journal of Agricultural Sciences

基　　金：国家“973”计划项目(2010CB100404);国家自然科学基金项目(30860005);云南省自然科学基金项目(2008CD124);云南省科技厅项目(2003C0017Q);云南省教育厅项目(02QY051);云南省学术技术带头人后备人才培养计划(2010CI031)

摘　　要：以分离自鳞翅目甜菜夜蛾[Spodoptera exigua(Hübner)]、斜纹夜蛾[Prodenia litura(Fabriciu)]和银纹夜蛾(Argyrogrammaagnate)罹病虫体的3个莱氏野村菌(Nomuraea rileyi)菌株NY-LE01、NY-PL02和NY-AA00为材料,研究了各菌株的生长速度、产孢量、萌发率、抗旱力及对甜菜夜蛾3龄幼虫的毒力。结果表明,菌株NY-LE01菌落生长速度、产孢量、培养24 h的孢子平均萌发率及抗旱能力明显高于NY-PL02和NY-AA00菌株。菌株NY-LE01、NY-PL02和NY-AA00对甜菜夜蛾幼虫的侵染致病毒力回归方程分别为Y=2.53+0.46X(r=0.936),Y=3.67+0.21X(r=0.942),Y=2.78+0.31X(r=0.892);各菌株对甜菜夜蛾幼虫的LC50(孢子/mL)分别为(2.342±0.132)×105、(2.154±0.215)×106和(1.450±0.21)×107孢子/mL。在1.26×105～1.26×108孢子/mL接种处理后,各菌株对甜菜夜蛾幼虫的LT50估计值范围分别为5.6～7.8、6.2～9.9和7.5～10.3 d。表明菌株NY-LE01对甜菜夜蛾具有较强的毒力,可作为甜菜夜蛾生防制剂开发的菌株。The colony growth rate,sporulation,germination in 24 h and drought-resistance of Nomuraea rileyi strains（NY-LE01,NY-PL02,NY-AA00） and their virulence against the larvae of Spodoptera exigua（Hübner）（Lepidoptera： Noctuidae） were evaluated under laboratory conditions. Three isolates were from Spodoptera exigua（Hübner）,Prodenia litura（Fabriciu） and Argyrogramma agnata.And the growth rate,sporulation and germination in 24 h of the strain of NY-LE01 was significantly higher than that of NY-PL02 and NY-AA00.The virulence regression equation of the strain NY-LE01,NY-PL02 and NY-AA00 against the larvae of S.exigua was Y=2.53＋0.46X（r=0.936）,Y=3.67＋0.21X（r=0.942）,Y=2.78＋0.31X（r=0.892）,respectively.The LC50 value was（2.342±0.132）×105,（2.154±0.215）×106 and（1.450±0.21）×107conidia/mL,respectively. The estimated LT50 value was 5.6-7.8,6.2-9.9 and 7.5-10.3 d,respectively under the inoculation concentration of 1.26×105-1.26×108conidia/mL.All the results showed that the strain of NY-LE01 was more virulent against S.exigua and with a potentiality in biological control.

关 键 词：莱氏野村菌 甜菜夜蛾 生物学特性 生物测定 

分 类 号：S435.66[农业科学—农业昆虫与害虫防治]