悬浮培养中提高川贝母鳞茎生物量的氨基酸前体组合配方优化  

Amino Acids Precusor Optimization of Biomass of Suspension Cultured Fritillaria cirrhosa D.Don Bulb

作　　者：王跃华[1] 朱丹青[1] 代勇 何玉红[1] 徐世军 邓启花[1] 王晓蓉 

机构地区：[1]成都大学生物产业学院,四川成都610016 [2]成都恩威集团(投资)有限公司,四川成都610041

出　　处：《西南农业学报》2011年第5期1908-1911,共4页Southwest China Journal of Agricultural Sciences

基　　金：四川省科技支撑计划(2010SZ0007);四川省中医药管理局(2010-79)

摘　　要：通过正交试验筛选快速提高川贝母鳞茎生物量的氨基酸前体组合配方,为工业化大规模生产名贵药材川贝母奠定基础,以川贝母小鳞茎为悬浮培养外植体,利用正交试验研究在川贝母小鳞茎悬浮培养中添加不同氨酸前体观察其对生物量的影响。结果表明,川贝母小鳞茎在添加由0.60 mmol.L-1酪氨酸、0.60 mmol.L-1苯丙氨酸和0.60 mmol.L-1色氨酸组合的悬浮培养液中培养25 d,其鳞茎的生物量提高最大可达3.5倍。The aim of the study was to screen the amino acids precusor formula that could improve the biomass of the suspension cultured Fritillaria cirrhosa D.Don Bulb to lay a foundation of industrialized mass production of Fritillaria cirrhosa D.Don Bulb.Taking Fritillaria cirrhosa D.Don Bulb bulblets as explants,the effect of different the amino acids precusor on the biomass was studied.The results showed that the biomass of bulblets that were cultured in suspension medium with 0.60 mmol·L-1 tyrosine,0.60 mmol·L-1 phenylalanine and 0.60 mmol·L-1 tryptophan after 25 d was increased by 3.5 times.

关 键 词：正交试验 川贝母 悬浮培养 氨基酸前体 

分 类 号：S567.231[农业科学—中草药栽培]