益气养阴解毒方抑制Lewis肺癌小鼠移植瘤生长及诱导移植瘤细胞凋亡的实验研究  

Inhibition of transplanted tumor growth and inducing of apoptosis by Yiqi Yangyin Jiedu Fang in mice with Lewis lung cancer

作　　者：张梅[1] 李平[1] 靳福鹏[1] 张锋利[1] 闫安[1] 杜静[1] 孙鑫[1] 

机构地区：[1]安徽医科大学附属省立医院,安徽230001

出　　处：《北京中医药大学学报》2011年第10期679-682,I0002,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家中医药管理局中医肿瘤病学重点学科资助项目(No.国中医药发[2009]30号)

摘　　要：目的研究益气养阴解毒方对Lewis肺癌(LLC)小鼠移植瘤生长及细胞凋亡的影响。方法复制C57BL/6小鼠Lewis肺癌模型,将接种Lewis肺癌的C57BL/6小鼠随机分为6组:对照组(A组)、益气养阴解毒方低剂量组(B组)、中剂量组(C组)、高剂量组(D组)、顺铂(DDP)组(E组)、益气养阴解毒方联合DDP组(F组)。每组10只,接种次日开始给药,中药连续灌胃26 d,DDP隔日腹腔注射,观察移植瘤生长情况,接种后第28天处死动物,称各组瘤重,计算抑瘤率;免疫组织化学法检测凋亡相关蛋白胱天蛋白酶-3(Caspase-3)表达;TUNEL法检测细胞凋亡指数。结果 B、C、D组对肿瘤均有较明显抑制作用,抑瘤率分别为19.30%、32.83%、34.59%,瘤重低于对照组,差异有显著性(P<0.01),F组抑瘤率达83.71%,与其他各组比较,差异有显著性(P<0.01);A组Caspase-3表达阴性,B、C、D组表达阳性,且与剂量成正相关(P<0.05),F组表达最强,与其他各组比较,差异显著(P<0.01)。TUNEL法检测示:中药组对移植瘤细胞的凋亡有促进作用,与DDP联合,凋亡促进作用增强(P<0.01)。结论益气养阴解毒方对Lewis肺癌小鼠移植瘤具有生长抑制作用,其作用机制可能与促进Caspase-3表达,诱导细胞凋亡有关,与DDP联合,这种作用增强。Objective To study the influences of Yiqi Yangyin Jiedu Fang on the growth and apoptosis of transplanted tumor in mice with Lewis lung cancer （LLC）. Methods The LLC model was established in C57BL/6 mice and then all mice after inoculating LLC were randomly divided into 6 groups, including the control group （ A）, group of low-dose Yiqi Yangyin Jiedu Fang （ B group）, group of mid-dose Yiqi Yangyin Jiedu Fang （C group）, group of high-dose Yiqi Yangyin Jiedu Fang （D group）, cisplatin （DDP） group （E group） and group of Yiqi Yangyin Jiedu Fang combining DDP （F group, each n = 10）. In the next day after inoculation Yiqi Yangyin Jiedu Fang were given to mice intragastrically for 26 days and DDP was injected intraperitoneally every other day. The growth of the transplanted tumor was observed, and the mice were killed on the 28th day for weighing tumor and calculating tumor inhibition rate. The expression of Caspase-3 （apoptosis-related protein） was detected by using immunohistochemistry, and apoptosis index, by using TUNEL. Results In B, C and D groups, the tumor was significantly inhibited, the tumor inhibition rate was, respectively, 19.30%, 32.83% and 34.59%, and tumor weight was lower than that in the control group （P 〈0.01 ）. The tumor inhibition rate reached 83.71% in F group with a significant difference compared with other groups （ P 〈 O. 01 ）. The expression of Caspase-3 was negative in A group, positive in B, C and D groups, which was correlated to dose （P 〈 0.05 ）, and the strongest in F group compared with other groups （P 〈 0.01 ）. The detecting of TUNEL showed that Chinese medicinal improved the apoptosis of transplanted tumor cells and this effect was promoted after Chinese medicinal combining with DDP （P 〈0.01 ）. Conclusion Yiqi Yangyin Jiedu Fang has an inhibitory effect on the growth of transplanted tumor in mice with LLC. The mechanism may be related to that Chinese medicinal can improve the expression of Caspase-3 and induce apoptosis, an

关 键 词：益气养阴解毒方 LEWIS肺癌 细胞凋亡 胱天蛋白酶-3 小鼠 

分 类 号：R285.5[医药卫生—中药学]