肺腺癌A549细胞株GDNF、BDNF、NT3和NT4的表达  

Expression of GDNF,BDNF,NT3 and NT4 in Human Lung Adenocarcinoma Cell Lines A549

作　　者：岳倩宇[1] 谭燕[1] 袁兵[1] 王盛兰[1] 王殿华[2] 张云辉[1] 

机构地区：[1]云南省第一人民医院呼吸内科,云南昆明650000 [2]昆明医学院药学院暨云南省天然药物药理重点实验室,云南昆明650031

出　　处：《昆明医学院学报》2011年第8期46-49,共4页Journal of Kunming Medical College

摘　　要：目的探讨神经营养因子在肺腺癌细胞株A549中的表达及临床意义.方法体外培养肺腺癌A549细胞株,观察细胞形态变化.取代培养后3 d的A549细胞,用免疫组化检测神经营养因子GDNF、BD-NF、NT3和NT4在肺腺癌细胞株A549的表达.结果神经营养因子GDNF、BDNF、NT3和NT4在部分肺腺癌细胞株A549中表达.结论神经营养因子GDNF、BDNF、NT3和NT4可能在肺癌的增殖中发挥作用.Objective To investigate the expression of neurotrophic factors（GDNF,BDNF,NT3,NT4）in human lung adenocarcinoma cell lines A549 and explore the functional significance.Method After cell lines A549 were cultured and morphological changes were observed,the expression of neurotrophic factors GDNF,BDNF,NT3 and NT4 were detected by immunohistochemistry in cell lines A549 on 3 days.Result Neurotrophic factors GDNF,BDNF,NT3and NT4 were expressed in part of cell lines A549.Conclusion The neurotrophic factors GDNF,BDNF,NT3 and NT4 may play a role in the proliferation of lung cancer cells.

关 键 词：A549 神经营养因子 肺腺癌 

分 类 号：R563.9[医药卫生—呼吸系统]