激光共聚焦显微镜观察小鼠卵母细胞及早期胚胎中微丝的分布  

Studies of the Microfilaments in Oocytes and Preimplantation Embryos by Confocal Laser Scanning Microscopy

作　　者：武迪迪[1] 高建[2] 孟峻[1] 刘超[1] 周末[2] 冯晨[1] 于秉治[1] 

机构地区：[1]中国医科大学基础医学院生化与分子生物学教研室,沈阳110001 [2]中国医科大学实验技术中心,沈阳110001

出　　处：《生殖与避孕》2011年第10期652-655,690,共5页Reproduction and Contraception

基　　金：国家自然科学基金青年基金(30800649);辽宁省高等学校科研项目(20060956)项目

摘　　要：目的:观察微丝在小鼠卵细胞及早期胚胎中的分布情况,为进一步明确微丝在受精卵发育中的作用机制打下基础。方法:采用免疫荧光化学法结合激光共聚焦显微技术对卵细胞的微丝分布进行观察。结果:在小鼠MII期卵母细胞中微丝分布于卵细胞的皮质区,集中在纺锤体处。在小鼠1-细胞期受精卵中微丝集中分布于卵细胞与极体的连接处。在2-细胞期受精卵中的微丝则集中分布在卵细胞的分裂沟处。用松弛素B(CB)解聚小鼠体内受精卵的微丝,细胞形态及分裂受到严重影响。结论:微丝在小鼠卵母细胞及早期胚胎中有着独特的分布,其可能参与多种功能。Objective：To observe the distribution of the microfilaments in oocyte and preimplantation mouse embryos.Methods：The distribution of the microfilaments in oocyte and preimplantation mouse embryos were observed by using immunofluorescent staining and confocal laser scanning microscopy （LCSM）.Results：In MII oocytes,microfilaments were observed beneath the plasma membrane and in whole cytoplasm of an oocyte,mainly existed in the meiotic spindle.The microfilaments appeared around the pole body in the 1-cell stage embryos.In 2-cell stage embryos,microfilaments were observed in the split channel.Conclusion：The distribution of microfilaments in MII oocytes and early embryos behaved different characteristics and regularities,suggesting it may participate in the control of processes of developmental processes.

关 键 词：微丝 小鼠卵母细胞 胚胎 免疫荧光技术 激光扫描共聚焦显微镜(LCSM) 

分 类 号：R339.22[医药卫生—人体生理学]