耳前入路小型猪耳蜗内电位的测定的研究  

A new approach to endocochlear potential measurement in mini pigs

作　　者：任丽丽[1,3] 郭德强[3] 陈磊[2] 郭维维[1] 李宁[2] 杨仕明[1] 

机构地区：[1]解放军总医院耳鼻咽喉头颈外科医院解放军耳鼻咽喉研究所,北京100853 [2]中国农业大学,农业生物技术国家重点实验室,北京100193 [3]解放军第148医院五官科,淄博255300

出　　处：《中华耳科学杂志》2011年第3期254-257,共4页Chinese Journal of Otology

基　　金：国家973计划重大科学问题导向项目(2011CBA01000);国家863计划(2007AA02Z150)

摘　　要：目的研究正常小型猪中耳及内耳解剖及耳前入路耳蜗内电位测定的可行性,为以小型猪作为动物模型的内耳数据测定及内耳疾病治疗提供实验基础和解剖学依据。方法 10只正常1周龄贵州香猪,麻醉后测量ABR确定听力正常,测听后分为2组,实验组(7只),以耳前入路进行中耳及内耳解剖,对照组(3只)以腹侧入路进行解剖。充分暴露耳蜗。分别测量每只小型猪左侧耳蜗内电位,观察正常小型猪的耳蜗内电位的范围。术后1天观察小型猪的基本生物学特征。结果实验组小型猪7耳中4耳测出稳定的耳蜗内电位;对照组3耳仅有1耳测出。术后1天实验组小型猪生命体征均平稳,正常饮食;对照组中1只术中死亡,其余术后状态欠佳。结论对于小型猪而言,耳前入路操作简单、损伤小、易于暴露圆窗龛,能够顺利完成耳蜗内电位的测定。Objective To describe a pre-auricular endocochlear potential measurement in mini pigs. Methods surgical approach to access the middle and inner ear for The left ear of 10 one-week old normal mini pigs were used in the study. The bulla was accessed via a pre-auricular approach for endocochlear potential measurement in 7 pigs （the experiment group）, and via aventrolateral approach in 3 pigs （the control group）, The conditions of the animals during the first post-experiment 24 hours were observed. ResuIts One animal in the control group died during surgery. The preauricular approach improved protection and preservation of relevant nervous and vascular elements including the facial nerve and carotid artery. Conclusion The pre-auricular approach can be used for endocochlear potential recording with improved nerve and artery preservation.

关 键 词：小型猪 内耳解剖 耳蜗内电位 

分 类 号：R322.923[医药卫生—人体解剖和组织胚胎学] R338.7[医药卫生—基础医学]