胆固醇息肉病人胆囊黏膜ABCG1表达的研究  

Expression of ABCG1 in gallbladder mucosa in patients with cholesterol polyps

作　　者：李成强[1,2,3] 韩天权[1,3] 蒋兆彦[1,3] 焦大海[4] 蔡劬[3] 蒋渝[1,2] 郑民华[1,2] 王明亮[1,2] 张圣道[1,3] 

机构地区：[1]上海交通大学医学院附属瑞金医院外科,上海200025 [2]上海交通大学医学院附属瑞金医院上海市微创外科临床医学中心,上海200025 [3]上海交通大学医学院附属瑞金医院上海消化外科研究所,上海200025 [4]安徽省阜阳市人民医院,安徽阜阳236000

出　　处：《外科理论与实践》2011年第5期474-477,共4页Journal of Surgery Concepts & Practice

基　　金：国家自然科学基金(30672042;30700310)

摘　　要：目的:探讨ABCG1基因在胆囊黏膜中的表达与胆固醇息肉病的关系。方法:采集15例胆囊胆固醇息肉病病人和8例无息肉无结石之对照组的胆囊黏膜和胆汁。测定胆汁胆固醇、胆汁酸和磷脂含量;real-time PCR测定胆囊黏膜中ABCG1 mRNA的表达;Western印迹法检测胆囊黏膜ABCG1之蛋白表达。结果:胆固醇息肉组胆汁胆固醇饱和指数、ABCG1mRNA表达均较对照组升高(P<0.05);息肉组中胆囊黏膜ABCG1在蛋白水平较对照组同样存在高表达。结论:胆固醇息肉病人之胆囊黏膜ABCG1基因及蛋白高表达,可能在胆固醇息肉疾病的发生、发展中起一定作用。Objective To explore the relationship between the mRNA expression of ABCG1 in gallbladder mucosa and the occurrence of cholesterol polyps.Methods Studies were made in 15 patients with cholesterol polyps and 8 patients without cholesterol polyps and gallstone.The biliary cholesterol,total bile acids and phospholipids were measured by kits,and the expression of ABCG1 mRNA detected by real-time PCR,the ABCG1 protein was detected by Western blotting.Results Cholesterol saturation index was increased in patients with cholesterol polyps（P0.05）.The ABCG1 mRNA expression in the cholesterol polyps group was higher than in the controls,and the ABCG1 protein level was also elevated in the cholesterol polyps group.Conclusions The expression of ABCG1 mRNA and protein were increased in the cholesterol polyps patients,indicating that ABCG1 may have some effects on the genesis and progression of gallbladder cholesterol polyps.

关 键 词：胆固醇息肉 胆囊 ABCG1 

分 类 号：R657.4[医药卫生—外科学]