龟足成体的5种消化酶活力  

Digestive enzyme activities in the alimentary tract of pedunculate cirripede Capitulum mitella

作　　者：林岗[1] 饶小珍[1] 陈宁[1] 郭世群[1] 许友勤[1] 

机构地区：[1]福建省发育与神经生物学重点实验室,福建师范大学生命科学学院,福建福州350108

出　　处：《热带海洋学报》2011年第5期88-94,共7页Journal of Tropical Oceanography

基　　金：福建省科技厅重点项目(2006N0066)

摘　　要：龟足是一种营养价值高,具有多种生理功能和保健作用的优质海产品,开展其消化生理的研究,可以为其规模化养殖提供理论依据。用酶学分析方法测定了龟足Capitulum mitella成体消化道5种消化酶的活力并探讨了温度、pH因子对5种消化酶活力的影响。结果表明,龟足的消化道能检测出类胰蛋白酶、胃蛋白酶、淀粉酶、纤维素酶和脂肪酶的活力,其中类胰蛋白酶活力最高,胃蛋白酶、淀粉酶、脂肪酶活力均较高,纤维素酶活力极低,类胰蛋白酶活力>胃蛋白酶活力,淀粉酶活力>纤维素酶活力。类胰蛋白酶、胃蛋白酶、淀粉酶的最适温度均为55℃,纤维素酶、脂肪酶的最适温度分别为45℃、35℃;类胰蛋白酶、胃蛋白酶、淀粉酶、纤维素酶、脂肪酶的最适pH分别为10.8、2.5、5.9、4.2—5.5、7—7.5。最适温度、pH下测得胰蛋白酶、胃蛋白酶、淀粉酶、纤维素酶、脂肪酶活力分别为:(267.07±13.69)U、(72.21±6.1)U、(28.62±1.6)U、(1.46±0.02)U、(65.24±1.8)U。淀粉酶(A)与类胰蛋白酶(T)活力的比值(A/T值)表明龟足是以动物食性为主的甲壳动物。Goose barnacle Capitulum mitella is a seafood with high nutrition and multiple biological and health benefits.To study its digestive functionality can provide theoretical foundation for its breeding.Five major digestive enzyme activities of the adult goose barnacle（Rostral-carinal length11 mm） and the effects of different temperature and pH values on activities of the digestive enzymes were studied by enzyme analysis.The results indicate that the activities of trypsin-like protease,pepsin,amylase,cellulase,and lipase were determined in alimentary tract and affected obviously by temperature and pH val-ues.The optimal temperatures for the activities of trypsin-like protease,pepsin,and amylase were 55℃,and 45℃ for cellu-lose,and 35℃ for lipase,respectively.The optimal pH values for trypsin-like protease,pepsin,and amylase were 10.8,2.5,and 5.9,respectively at 55℃,and those for cellulase and lipase were 4.2？5.5 at 35℃ and 7.0？7.5 at 45℃.The activities of five digestive enzymes were in an order from strong to weak： trypsin-like protease,pepsin,amylase,cellulase,and lipase in alimentary tract at the most favorable reacting temperature and pH.The low ratio of Amylase/Trypsi（A/T） indicates that Capitulum mitella is a kind of carnivorous crustacean.

关 键 词：龟足 消化酶活力 温度 PH 食性 

分 类 号：S917[农业科学—水产科学]