丽格海棠‘宣言’离体再生因素的优化  

OPTIMIZATION OF THE FATCTORS AFFECTING THE REGENERATION OF RIEGER BEGONIA ‘MANIFESTO’ IN VITRO CULTURE

作　　者：王海朋[1] 羿德磊[1] 段祖安[1] 崔为正[1] 王洪利[1] WANG Hai-peng;YI De-lei;DUAN Zu-an;CEI Wei-zheng;WANC Hong-li(Key lab of Agricultural Environment and Ecology,College of Forestry,Shandong Agricultural University,Taian 271018,China)

机构地区：[1]山东农业大学林学院农业生态与环境重点实验室,山东泰安271018

出　　处：《山东农业大学学报（自然科学版）》2012年第1期7-11,共5页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：现代农业产业技术体系建设专项(No.nycytx-27-gw304)

摘　　要：丽格海棠再生分化受到多种因子的影响,通过对丽格海棠离体再生影响因素的优化试验表明:以叶片为外植体,MS+6-BA 2.0 mg.L-1+NAA 0.2 mg.L-1最利于海棠的不定芽诱导;不定芽增殖分化的培养基为MS+1/2MS(Trace element)+6-BA 1.5 mg.L-1+NAA 0.1 mg.L-1+Sucrose 30 g.L-1;生根壮苗培养基为1/2MS+IAA 0.1 mg.L-1。The regeneration and differentiation of Rieger begonia was affected by lots of factors,this paper study on the optimization test of those factors and the rescts show the followings as explant with leaves,the culture MS＋6-BA 2.0 mg· L-1 ＋NAA 0.2 mg· L-1-1 is the best on induction buds;Besides,the medium best for regeneration is MS＋1/2MS（Trace element）＋6-BA 1.5 mg· L-1＋NAA 0.1 mg· L-1＋Sucrose 30 g· L-1;1/2MS ＋IAA 0.1 mg· L-1 is the fittest culture medium for seedling and rooting.

关 键 词：丽格海棠'宣言’ 组织培养 正交试验 

分 类 号：S722.3[农业科学—林木遗传育种]