乙醇回流法提取山豆根总生物碱的工艺研究  

Optimization of the Extraction Technology of Total Alkanoids from Radix Sophorae Tonkinensis with Ethanol Reflux Extraction Technology

作　　者：陆娟[1] 杨帆[1] 刘春明[1] 

机构地区：[1]长春师范学院化学学院,吉林长春130032

出　　处：《长春师范学院学报（自然科学版）》2011年第5期48-52,共5页Journal of Changchun Teachers College

基　　金：吉林省教育厅项目(吉教科合字2010379);长春师范学院资助项目([2010]第026号)

摘　　要：以山豆根为原料,利用乙醇回流法提取山豆根总生物碱,通过考察乙醇浓度、料液比、提取时间、提取次数四个单因素和L9(34)正交试验设计,对山豆根总生物碱的提取工艺进行优化。结果表明:影响山豆根总生物碱提取含量的各因素主次顺序为:乙醇浓度(A)>料液比(B)>提取时间(C)>提取次数(D),优选出的最佳提取工艺组合为:A2B2C3D2,即乙醇浓度为40%,料液比为1∶20,提取时间2h,提取2次。在此条件下,山豆根总生物碱的提取含量为16.8537 mg.g-1。The total alkanoids from radix sophome tonkinensis were extracted with reflux technology. The effects of ethanol concentration, material - liquid ratio, extraction time and extraction times were studied through single factor test. And the extraction technology of total alkaloids from radix sophorae tonkinensis was optimized by using L9 （3^4） orthogonal test. The resuits showed that according to the results of orthogonal test, the sequence of influential significance was as follows： ethanol concentration（A） 〉 material- liquid ratio（B） 〉 extraction time（C） 〉 extraction times（D）, the optimum technology of reflux extraction was that the ethanol concentration was 40% ,the ratio of material to liquid was 1：20,the time of extraction was 2h and the times of extraction was twice; the total alkaloids content of the extraction from radix sophorae tonkinensis was 16. 8537mg· mL^-1.

关 键 词：山豆根 总生物碱 提取工艺 

分 类 号：R286[医药卫生—中药学]