用于HPLC法测定多胺类化合物的4种荧光衍生试剂的合成及其性质  

Synthesis and Properties of Four Fluorescent Reagents for HPLC Determination of Polyamines

作　　者：许泳吉[1] 王永智[1] 

机构地区：[1]青岛科技大学化学与分子工程学院,山东青岛266042

出　　处：《青岛科技大学学报（自然科学版）》2011年第5期488-491,505,共5页Journal of Qingdao University of Science and Technology:Natural Science Edition

摘　　要：设计并合成了4种相对分子质量较小、疏水性较低的荧光衍生试剂:邻氨基苯甲酸酐、N-甲基邻氨基苯甲酸酐、2-氨基烟酸活性酯和水杨酸活性酯。系统研究了这些衍生试剂与己胺的反应性能,并合成了相应的衍生物标准品,测定了衍生物的荧光性质。结果表明:4种衍生试剂中邻氨基苯甲酸酐适合用作高效液相色谱荧光检测多胺的衍生试剂。Four fluorescent reagents, anthranilic acid anhydride, N-methyl-o-aminobenzoic acid anhydride, 2-aminonicotinic acid active ester and salicylic acid active ester, were designed and synthesized. The reaction properties between these reagents and hexylamine were studied. The corresponding standard derivatives were synthesized and the fluorescent properties of these derivatives were also measured. The results showed that anthranilic acid anhydride was a suitable fluorescent reagent for detection of polyamine by HPLC.

关 键 词：高效液相色谱 荧光衍生试剂 多胺 

分 类 号：O657.7[理学—分析化学]