氧化苦参碱诱导人胃癌细胞凋亡的实验研究  

Experiment study on apoptosis of human gastric carcinoma cells induced by Oxymatrine

作　　者：刘粉霞[1] 徐玉芳[2] 孙宁[2] 

机构地区：[1]河南省中医院病理科,河南郑州450002 [2]河南中医学院,河南郑州450008

出　　处：《中国医药导报》2011年第31期27-28,32,共3页China Medical Herald

基　　金：河南省重点科技攻关计划(项目编号:082102310013);郑州市科技攻关项目(项目编号:0910SGYS33390-1)

摘　　要：目的:研究氧化苦参碱诱导人胃癌MGC-803细胞裸鼠移植瘤细胞凋亡的作用机制。方法:制备人胃癌MGC-803细胞裸鼠移植瘤模型,将其随机分为阴性对照组、阳性对照组及氧化苦参碱小、中、大剂量组,观察氧化苦参碱对人胃癌裸鼠移植瘤的抑制作用。流式细胞仪检测移植瘤细胞Bcl-2、Bax的表达。结果:氧化苦参碱能有效抑制裸鼠移植瘤的生长,与阴性对照组比较,其大剂量组肿瘤抑制作用明显,平均抑制率为43.20%;流式细胞仪检测显示氧化苦参碱组裸鼠移植瘤细胞Bcl-2表达降低,而Bax表达升高,以中剂量氧化苦参碱诱导细胞凋亡最明显,移植瘤细胞Bcl-2表达为(339.19±42.34),与阴性对照组(492.31±36.19)比较,差异有统计学意义(P<0.05);Bax表达为(554.74±26.13),与阴性对照组(397.48±18.36)比较,差异有高度统计学意义(P<0.01)。结论:氧化苦参碱对人胃癌MGC-803细胞裸鼠移植瘤生长有一定的抑制作用,且其机制与下调细胞Bcl-2的表达、上调Bax的表达,从而诱导肿瘤细胞凋亡有关。Objective： To study the apoptosis mechanisms of Oxymatrine on transplanted MGC-803 cells of human gastric carcinoma in nude mice. Methods： The models of human gastric carcinoma cell line MGC-803 in nude mice were copied. The mice were grouped randomly into five groups, the negative control group, the positive control group, the low, mid and high dosage Oxymatrine groups. The changes of the transplanted tumor growing were observed. FCM were applied to analyze the expression level of Bcl-2 and Bax in transplanted gastric carcinoma. Results： Oxymatrine could inhibit the growth of human gastric carcinoma in nude mice. Compared with the negative group, the high dosage Oxymatrine group had better effect, which tumor inhibition rate was 43.20%; FCM showed that the expressions of Bcl-2 were down-regulated while Bax were up-regulated in Oxymatrine groups. The mid dosage Oxymatrine was the proper dose to induce tumor cell apoptosis, the expression level of Bcl-2 was （339.19±42.34）, compared with the negative control group （492.31±36.19）, there was a significant difference （P〈0.05）; the expression level of Bax was （554.74±26.13）, compared with the negative control group （397.48± 18.36）, there was a significant difference （P〈0.01）. Conclusion： Oxymatrine is effective in treating transplanted human gastric carcinoma cell line MGC-803 in nude mice, which mechanism may be inducing tumor cell apoptosis, its molecular mechanisms may be through down-regulation of Bcl-2 expression and up-regulation of Bax expression.

关 键 词：氧化苦参碱 胃癌 凋亡 

分 类 号：R273[医药卫生—中西医结合]