Isolation of Lysozyme from Chicken Egg White Using Polyacrylamide-based Cation-exchange Cryogel  

作　　者：晏禄丁 沈绍传 贠军贤 姚克俭 

机构地区：[1]State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310032, China

出　　处：《Chinese Journal of Chemical Engineering》2011年第5期876-880,共5页中国化学工程学报（英文版）

基　　金：Supported by the National lqatural Science Foundation of China （21036005, 20876145）, the Science and Technology Cooperation Project between China-Europe Country＇s Governments from the Ministry of Science and Technology of China （1017） and the Natural Science Foundation of Zhejiang Provincial （Y4080326）.

摘　　要：An effective cation-exchange chromatographic method for lysozyme isolation from chicken egg white is presented, using supermacroporous cryogel grafted with sulfo functional groups. The chromatographic processes were carried out by one-step and sequential elution, respectively. Sodium phosphate buffer （pH 7.8） containing different concentrations of NaC1 is used as elution agent. The corresponding breakthrough characteristics and elution behaviors in the cryogel bed were investigated and analyzed. Purity of lysozyme in the elution effluent was assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）. The maximum purity of the obtained lysozyme was about 96%, and the cryogel is demonstrated as a potential separation medium for purification of high-purit lysozyme from chicken egg white.有效阳离子交换为从鸡肉鸡蛋白色的溶解酵素隔离的色析法的方法被介绍，用有 sulfo 的 supermacroporous cryogel grafted 功能的组。色析法的过程被一步舞和顺序的 elution 分别地执行。钠磷酸盐缓冲区(pH 7.8 ) 包含 NaCl 的不同集中被用作 elution 代理人。在 cryogel 床上的相应突破特征和 elution 行为被调查并且分析。在 elution 自河的溶解酵素的纯净是由钠 dodecyl sulfate-polyacrylamide 胶化电气泳动(SDS 页) 的 assayed。获得的溶解酵素的最大的纯净是大约 96% ，并且 cryogel 从鸡鸡蛋白色作为高纯净的溶解酵素的纯化的潜在的分离媒介被表明。

关 键 词：cation-exchange cryogel.iysozyme ISOLATION sequential elution chicken egg white 

分 类 号：Q503[生物学—生物化学] TQ613[化学工程—精细化工]