人型耶氏肺孢子虫姬姆萨氏染色形态表达  

Giemsa's dyeing form expression of human Pneumocystis jirovecii

作　　者：陈敬捷 李勇 何晗 苏凌松 刘春明[3] 秦松树 唐柳生 李世立 胡宏波 

机构地区：[1]广西壮族自治区龙潭医院检验科,柳州545005 [2]广西壮族自治区龙潭医院艾滋病科,柳州545005 [3]柳州市中医院检验科 [4]柳州市肿瘤医院检验科 [5]柳铁中心医院检验科

出　　处：《中国临床新医学》2011年第10期913-916,共4页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

基　　金：国家十一五科技重大专项资金资助项目(编号:2008ZX10005-003);广西卫生厅自筹经费科研课题(编号:Z2007030)

摘　　要：目的研究人型耶氏肺孢子虫姬姆萨氏染色形态。方法采用姬姆萨氏染色法涂片染色痰液和肺泡灌洗液孢子虫,在油镜下观查形态。结果痰液人型孢子虫大小约1.0～7.5μm,包囊圆形或椭圆形,包囊囊壁染成淡蓝色,囊内小体染成紫红或紫蓝色;滋养体胞浆染成淡蓝色,内容细胞质染成紫红或紫蓝色;包囊可单个、几个、散在、成群或串状存在,成群排列常伴不同发育阶段孢囊和滋养体,虫体大小差异较大,形态多样,成熟典型的包囊很少,大多是不成熟包囊或滋养体。结论人型肺孢子虫姬姆萨氏染色形态较动物孢子虫形态更显大小不均、形态多样,常以不成熟包囊和滋养体在片中表达。Objective To study Giemsa＇ s dyeing form of human pneumocystis jirovecii（PJ）. Methods Gi- emsa staining method was used for smearing and dyeing of sputum and BALF PJ. The PJ forms were observed under microscope with oil immersion lens. Results PJ in sputum showed about the size of 1.0 - 7.5 μm. Its cysts were round or oval. The cyst wall was light blue. Intracystie bodies were purple or purplish blue. Trophozoite cytoplasm was light blue. Its cytoplasm was purple or purplish blue. The cysts can be single, several, scattered, arranged in groups, or dusters. In the cysts arranged in groups there were different development stages of trophozoites. The difference of bug body size was bigger. Bug body form was diversiform. Typical mature cysts were very rare. Immature cysts and trophozoites were in the majority. Conclusion Giemsa＇ s dyeing form of hunan PJ have more types than animal PJ. Often in immature cysts and trophozoites are in the majority in the film expression.

关 键 词：耶氏肺孢子虫(菌) 姬姆萨氏染色 染色形态 

分 类 号：R382.3[医药卫生—医学寄生虫学]