306份血标本乙肝5项指标定量与定性检测结果分析  

Analyze the results of quantitative and qualitative detection in 360 pieces of blood specimens of fine items of hepatitis B

作　　者：赵平[1] 赵成桂[2] 张臣[2] 孙宏伟[2] 

机构地区：[1]大连大学职业技术学院检验教研室,辽宁大连116001 [2]东南大学附属中大医院检验科,江苏南京210009

出　　处：《现代医学》2011年第5期551-553,共3页Modern Medical Journal

摘　　要：目的:观察乙肝5项指标定量与定性检测结果的差异及临床意义。方法:对306份血标本分别采用ELISA法和化学发光法检测乙肝5项指标,并对结果进行系统分析。结果:ELISA法和化学发光法分别检测出7种和13种模式,两种方法的一致率分别为乙肝病毒表面抗原(HBsAg)98.8%,乙肝病毒表面抗体(HBsAb)82.4%,乙肝病毒e抗原(HBeAg)96.4%,乙肝病毒e抗体(HBeAb)78.2%和乙肝病毒核心抗体(HBcAb)59.2%;化学发光法检测HBeAb和HBcAb的阳性率显著高于ELISA法(P<0.001)。结论:定量检测乙肝5项指标敏感性高于常规定性检测方法;同一份标本用两种方法检测可出现不同的结果模式,应引起临床重视。Objective： To investigate and compare differences between quantitative and qualitative determination of hepatitis B markers.Methods： Three hundred and six of samples were detected by two methods and the data were analysed by using statistics software.Results： Seven and thirteen models of hepatitis B markers were found by using enzyme-linked immunosorbent assay（ELISA） and chemiluminescent immunoassay（CLIA）.The concordance rates of HBsAg,HBsAb,HBeAg,HBeAb and HBcAb were 98.8%,82.4%,96.4%,78.2% and 59.2%,respectively.There were significantly differences on the positive rates of HBeAb and HBcAb detected by ELISA,as compared with that of CLIA（P0.001）.Conclusion： The sensitivity of CLIA is higher than that of ELISA.The different models of hepatitis B markers appear when the same sample was determined by using ELISA and CLIA.

关 键 词：乙肝5项指标 定量检测 定性检测 

分 类 号：R512.62[医药卫生—内科学]