两种不同淋巴细胞分离液对脐带血单核细胞的提取及后期诱导培养CIK细胞的影响  

Effect of Two Different lymphocyte Separatory Liquids on the Extraction of Umbilical Cord Blood mononuclear cells and Later Induction Culture of CIK Cells

作　　者：张校通 赵令卉 王小燕 王翔 申重阳 郑榆坤 

机构地区：[1]成都清科生物科技有限公司,成都610000

出　　处：《生命科学仪器》2011年第5期49-51,共3页Life Science Instruments

摘　　要：目的:研究两种不同的淋巴细胞分离液对脐带血单核细胞的分离效果及后期对CIK细胞的诱导培养的影响。方法:分别使用国产TBD以及进口GE healthcare牌淋巴细胞分离液分离提取脐带血单核细胞,用悬浮细胞培养法诱导培养脐带血单核细胞形成CIK细胞,再用血球计数板及台盼兰染色法检测细胞密度及活率。结果:国产的TBD牌所分离的单核细胞分层较清楚,并且方便提取,而使用进口GE healthcare的淋巴细胞分离液提取的单核细胞数量较多,但经过后期诱导培养CIK细胞发现两种分离液提取的细胞培养到后期数量逐渐接近。结论:国产TBD牌淋巴细胞分离液在用于脐带血淋巴细胞分离提取时较进口GE healthcare牌经济,并且细胞提取方便,后期培养效果无明显差别。Objective：To compare the result of umbilical cord blood mononuclear cells extracted by two different lymphocyte separation liquids,and the later effect on CIK culture.Methods：The improved methods were used to extract mononuclear cells from umbilical cord blood.Then the mononuclear cells were inducted and cultured to CIK cells.Lastly,the blood corpuscle count plate and trypan blue were used to determination the density and live rate of CIK cells.Results：Compared with the GE healthcare,the quantity of mononuclear cells separated by TBD was less,while the stratification was clearer and more convenient to extract.Besides,when the cells cultured to CIK later,their density and live rate were close gradually.Conclusion：The TBD lymphocyte separation fluid was more economical and convenient than GE healthcare when used to separate mononuclear cells from umbilical cord blood;in addition,there were no significant difference on later culture.

关 键 词：淋巴细胞分离液 脐带血 单核细胞 CIK细胞 

分 类 号：Q959.223.5[生物学—动物学]