枇杷花镇咳活性部位筛选  

Effective Part Screening of the Flower of Eriobofryajaponica on Antitussive Activities

作　　者：梁慧[1] 陈晓芳[1] 刘春宇[1] 

机构地区：[1]苏州大学医学部药学院,江苏苏州215123

出　　处：《中国血液流变学杂志》2011年第3期411-412,427,共3页Chinese Journal of Hemorheology

摘　　要：目的 探寻枇杷花95%乙醇提取物 （Flower of Eriobotrya japonica,FEJ） 的止咳活性部位.方法 枇杷花醇提物依次用石油醚、氯仿、乙酸乙酯及水层萃取,得4个部位.分别给予小鼠灌胃,采用氨水喷雾法记录小鼠3min咳嗽次数和咳嗽潜伏期.结果 FEJ氯仿萃取部位、乙酸乙酯萃取部位和水层高剂量组明显减少小鼠咳嗽次数和咳嗽潜伏期（P＜0.01）.结论 FEJ有镇咳作用,其氯仿和乙酸乙酯萃取部位及水层均有显著的镇咳作用,尤其是氯仿和水层高剂量组效果最好.Objective To study the effective part screening of the ethanol extract from Flower of Eriobotrya japonica（FEJ） on the antitussive activities for clinical application. Methods The mice were administrated with four factions which were separated with petroleum ether, chloroform,ethyl acetate,water layer from FEJ.Using cough model induced Citric acid,the incubation period of cough time and 5 minutes cough in guinea-pig were recorded from beginning citric spray to presenting cough;With cough model induced ammonia water,the incubation period of cough time and 3 minutes cough in mice were recorded from beginning ammonia spray. Results FEJ extracted with chloroform,ethyl acetate,the high dose of water layer from FEJ significantly reduces the cough of guinea pigs and mice obviously（P 〈 0.01）. Conclusion FEJ has antitussive actions on animal experiments.Extracted chloroform,ethyl acetate,water layer from FEJ have significantly antitussive action,especially the antitussive effect is the best in the highest dosage of chloroform and water layer groups.

关 键 词：枇杷花 镇咳 活性筛选 

分 类 号：R965.1[医药卫生—药理学]