NP-HPLC法测定三七药材中总皂苷含量  

Determination of Total Saponins in Panax Notoginseng by NP-HPLC

作　　者：高瑛[1] 翟永松[1] 杜守颖[1] 徐冰[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中国药品标准》2011年第5期346-349,共4页Drug Standards of China

基　　金：国家"重大新药创制"科技重大专项(2009zx09502-008);国家"十一五"科技支撑计划(2006BA106A17)

摘　　要：目的:建立正相高效液相色谱法同时测定三七药材中人参皂苷Rg1,Re,Rb1,三七皂苷R1 4种成分的含量测定方法,并对不同规格三七药材中三七总皂苷的含量进行比较。方法:采用正相高效液相色谱法,色谱柱用Phenomenex NH2色谱柱(4.6mm×250 mm,5μm),流动相为乙腈-水(82∶18),等度洗脱,检测波长203 nm,流速1 mL.min-1,柱温30℃。结果:人参皂苷Rg1,Re,Rb1,三七皂苷R14种成分30 min内即可分析完全,方法学考察各项结果均符合要求。不同规格三七药材中三七总皂苷的含量存在差别,总皂苷含量趋势为40头>60头>80头>120头>无数头。结论:该方法灵敏度高,快速准确,专属性强,重现性好,可用于三七药材的质量评价。Objective： An NP-HPLC method was developed for determination of ginsenoside Rg1 , ginsenoside Re, ginsenoside Rb1 , notoginsenoside R1 , and with the four components to determine and compare the total saponins in different specification of Panax Notoginseng. Methods： The NP-HPLC system consisting of Phenomenex-NH2 column（4. 6 mm x 250 mm,5 μm）and a mixture of acetonitrile-water（82： 18） as the mobile phase was adopted. The flow rate was 1.0 mL· min-1 , the UV detection was set at 203nm and the oven was 30 ℃. Results： The ginsenosides RgI , Re, Rb1 , notoginsenoside RI could be analyzed in 30min, and the results of methodology was good. The total saponins are different in different specification of Panax Notoginseng. The content trend is ： 40 tou 〉 60 tou 〉 80 tou 〉 120 tou 〉 innumerable tou. Conclusion： The method is sensitive, accurate, simple and repeatable; it can be used as the quality control for Panax Notoginseng.

关 键 词：三七 正相高效液相色谱法 人参皂苷RG1 人参皂苷RE 人参皂苷RB1 三七皂苷R1 

分 类 号：R921.2[医药卫生—药学]