高良姜道地药材的高相液相色谱指纹图谱研究  

Study on HPLC Fingerprint of Alpinia officinarum

作　　者：邓亦峰[1] 冯丽娜[1] 罗辉[1] 

机构地区：[1]广东医学院/广东省天然药物研究与开发重点实验室,广东湛江524023

出　　处：《中药材》2011年第9期1351-1355,共5页Journal of Chinese Medicinal Materials

基　　金：广东省建设中医药强省科研基金资助项目(No.2060044)

摘　　要：目的:采用高效液相色谱法建立了广东徐闻高良姜药材的指纹图谱。方法:采用液相色谱-质谱联用(LC-MS)进行峰识别和分离度判断,获得优化的色谱分离条件:Shim-pack VP-ODS柱(2.0 mm×250 mm,5μm),流动相A为0.1%HAC水溶液,流动相B为15%乙腈+40%甲醇+45%四氢呋喃,程序梯度洗脱;流速0.2 mL/min,柱温35℃,检测波长280 nm。结果:对所采集的10批高良姜药材进行了测定,建立了高良姜的高效液相色谱指纹图谱,确定了10个共有峰及其相对保留时间和相对峰面积。结论:本分析方法稳定、可靠,可用于高良姜药材质量的控制和鉴别。Objective： To establish the chromatography fingerprint of Alpinia officinarum by HPLC.Methods： An optimum HPLC conditions which were obtained under the assessment of LC-MS were as follows： Shim-pack VP-ODS column（2.0 mm×250 mm,5μm）,0.1%HAc aqueous solution as phase A,15%Acetonitrile∶40%Methanol∶45%Tetrafuran as phase B, the flow rate was 0.20 mL/min,column temperature was 35℃ and UV detector was set at 280 nm.Results： The HPLC fingerprint of Alpinia officinarum was established,the consensus 10 peaks and their relative retention times along with the ranges of relative area were determined.Conclusion： The method is reliable and stable and can be used for the quality control and identification of Alpinia officinarum.

关 键 词：高良姜 指纹图谱 高效液相色谱 

分 类 号：R282.5[医药卫生—中药学]