淫羊藿多糖脂质体制备条件的优选  

Preparation Conditions Optimization of Epimedium Polysaccharide Liposome

作　　者：高焕[1] 高琪[1] 王德云[1] 范云鹏[1] 郭利伟[1] 赵晓娜[1] 

机构地区：[1]南京农业大学中兽医学研究室,江苏南京210095

出　　处：《中药材》2011年第9期1429-1433,共5页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金项目(30901085);教育部科学技术重点项目(108153)

摘　　要：目的:优选淫羊藿多糖脂质体(EPSL)制备的最佳条件。方法:采用薄膜分散超声法制备EPSL,以包封率和载药量为指标,以脂药比、膜材比、超声时间和载药温度为因素水平,用L9(34)正交试验对制备条件进行优选,并用鱼精蛋白法测定EPSL的包封率。结果:制备EPSL的最佳条件为:脂药比30∶1,膜材比4∶1,超声时间10 min,载药温度40℃。结论:采用最佳条件制备的EPSL,包封率和载药量较高。Objective: To optimize the preparation conditions of epimedium polysaccharide liposome(EPSL).Methods: EPSL was prepared by membrane distribution-supersound method.In preparation of EPSL test,an orthogonal L_9(34) test was used to optimize the preparation conditions,with four factors of ratio of drug to lipid,ratio of lecithin to cholesterol,ultrasonic time and water bath temperature and two indexes of encapsulation efficiency and drug-loading rate.The encapsulation efficiency of EPSL was determined by protamine method.Results: Optimal preparation condition of EPSL was that ratio of drug to lipid,ratio of lecithin to cholesterol,ultrasonic time and water bath temperature were 1∶30,4∶1,10 min and 40℃ respectively.Conclusion: EPSL prepared under Optimal conditions has the property of high encapsulation efficiency and drug-loading rate.

关 键 词：淫羊藿多糖脂质体 制备 正交试验 鱼精蛋白法 

分 类 号：R286.1[医药卫生—中药学]