不同浓度骨痹舒片含药血清对体外培养软骨细胞增殖的影响  

Effects of different concentrations of Gubishu containing serum on the proliferation of rabbit articular chondrocytes in vitro culture

作　　者：秦梦[1] 王和鸣[1] 娄玉钤 

机构地区：[1]福建中医药大学,福建福州350108 [2]河南风湿病医院

出　　处：《中国骨伤》2011年第10期841-844,共4页China Journal of Orthopaedics and Traumatology

摘　　要：目的:观察不同浓度骨痹舒片含药血清对体外培养的兔关节软骨细胞增殖的影响。方法:获取1月龄兔关节软骨细胞,随机分为空白组(正常兔血清)及骨痹舒片含药血清组,每组再分5%、10%、15%、20%4个浓度,共8组。分别于培养第1、3、5、7、9天用MTT法检测软骨细胞的增殖情况。结果:骨痹舒片含药血清组细胞呈血清浓度依赖型增殖。同一时间点各组间比较差异均有统计学意义(P<0.05),以20%组最佳;空白血清组中5%、10%组与20%组相比在各时间点差异均有统计学意义(P<0.05),15%组与20%组相比在第1、3、5、7天各时间点相比差异无统计学意义(P>0.05),在第9天时差异有统计学意义(P<0.05),以20%组最佳。20%骨痹舒片含药血清组与20%空白血清组相比,在第1、3、5、7天时差异有统计学意义(P<0.05),第9天差异无统计学意义(P>0.05)。结论:20%的骨痹舒片含药血清能显著促进软骨细胞的增殖,并将细胞的指数增长期提前至第3天。Objective：To investigate the effects of different concentrations of Gubishu containing serum on the proliferation of rabbit articular chondrocytes cultured in vitro.Methods：Articular chondrocytes were obtained from the cartilage of 1-month rabbit and cultured in vitro.They were randomly divided into 8 groups,blank and Gubishu groups in different concentrations（5%,10%,15%,20%）,MTT assay method was adopted to observe the influence of Gubishu containing serum with different concentrations to the proliferation of chondrocytes after incubated 1,3,5,7 and 9 days.Results：The proliferation of chondrocytes was dependent on the concentration in Gubishu groups.At same time point,there was significant value between every groups,20% concentration was greatest（P〈0.05）;There was significant differences between 5%,10% and 20% concentration of the blank groups at same time point（P〈0.05）,and was not between 15% and 20% concentration at the 1,3,5 and 7 days（P〉0.05）,20% concentration of the blank group was greatest.20% concentrations of Gubishu containing serum was significantly greater than 20% concentrations of blank group at the 1,3,5 and 7 days（P〈0.05）.Conclusion：20% concentrations of Gubishu containing serum can significantly increase the proliferation of chondrocytes,and bring the logarithmic growth period forward to the 3 day.

关 键 词：软骨细胞 细胞 培养的 中草药 细胞增殖 

分 类 号：R684.3[医药卫生—骨科学]