Scavenging Effect of Naoerkang(脑尔康) on Amyloid BetaPeptide Deposition in the Hippocampus in a Rat Model of Alzheimer's Disease  

作　　者：李玺 袁海峰 权乾坤 王建军 王宁宁 李明 

机构地区：[1]Department of Geriatrics the Second Affiliated Hospital,School of Medicine,Xi'an Jiaotong University [2]Department of Neurology the Second Affiliated Hospital,School of Medicine,Xi'an Jiaotong University [3]Research Center of Rehabilitation Science and Technology School of Life Science and Technology,Xi'an Jiaotong University [4]Department of Geriatrics Fuzhou General Hospital of Nanjing Military District

出　　处：《Chinese Journal of Integrative Medicine》2011年第11期847-853,共7页中国结合医学杂志（英文版）

基　　金：Supported by Tackle Key Problem in-Science and Technology Foundation of Shaanxi Province[No.2007K16-07(5)]

摘　　要：Objective： To observe the effect of a Chinese medicine compound, Naoerkang （脑尔康, NEK）, on amyloid-beta peptide （1-42; A 131-42） and matrix metalloproteinase-9 （MMP-9） expressions in the hippocampus of AIzheimer＇s disease （AD） model rats. Methods： A total of 48 male Sprague Dawley （SD） rats were randomly divided into normal control, untreated, and piracetam groups, and low-dose, medium-dose, and high-dose NEK groups, with 8 rats in each group. The 5-1μL aggregated Aβ1-42 （2μg/μL） were injected into both CA1 areas of the hippocampus in the rats to establish an AD model, whereas the normal control was treated with the same dose of normal saline. The rats in the NEK groups were treated with a high, medium, or low dose of NEK [60 g/（kg-d）, 30 g/（kg-d）, and 15 g/（kg.d）], respectively, intragastrically for 28 days; piracetam （0.375 g/kg, intragastrically） was consecutively administered in the piracetam group; and normal saline was applied in the normal control and untreated groups. A Y-maze test was used for behavioral study to test the learning and memory abilities. A 131-~ and MMP-9 expressions in the hippocampus was determined immunohistochemically, and the results were analyzed by image acquisition and an analysis system. Results： Aggregated A 131.42 induced obvious learning and memory dysfunction, as well as up-regulation of A 13 1-42 expression in the hippocampus. Compared with those in the normal control group, the learning and memory abilities of rats in the untreated group significantly decreased （P〈0.01）, and the expression of A β1-42 was significantly increased （P〈0.01）. Twenty-eight days after different treatments, compared with those in the untreated group, the learning and memory abilities of AD model rats in the piracetam, low-dose, medium-dose and high-dose NEK groups were significantly improved （P〈0.01 or P〈0.05）, and the expression of Aβ1-42 in the hippocampus decreased （P〈0.01 or P〈0.05）, and MMP-9 increased （P�Objective： To observe the effect of a Chinese medicine compound, Naoerkang （脑尔康, NEK）, on amyloid-beta peptide （1-42; A 131-42） and matrix metalloproteinase-9 （MMP-9） expressions in the hippocampus of AIzheimer＇s disease （AD） model rats. Methods： A total of 48 male Sprague Dawley （SD） rats were randomly divided into normal control, untreated, and piracetam groups, and low-dose, medium-dose, and high-dose NEK groups, with 8 rats in each group. The 5-1μL aggregated Aβ1-42 （2μg/μL） were injected into both CA1 areas of the hippocampus in the rats to establish an AD model, whereas the normal control was treated with the same dose of normal saline. The rats in the NEK groups were treated with a high, medium, or low dose of NEK [60 g/（kg-d）, 30 g/（kg-d）, and 15 g/（kg.d）], respectively, intragastrically for 28 days; piracetam （0.375 g/kg, intragastrically） was consecutively administered in the piracetam group; and normal saline was applied in the normal control and untreated groups. A Y-maze test was used for behavioral study to test the learning and memory abilities. A 131-~ and MMP-9 expressions in the hippocampus was determined immunohistochemically, and the results were analyzed by image acquisition and an analysis system. Results： Aggregated A 131.42 induced obvious learning and memory dysfunction, as well as up-regulation of A 13 1-42 expression in the hippocampus. Compared with those in the normal control group, the learning and memory abilities of rats in the untreated group significantly decreased （P〈0.01）, and the expression of A β1-42 was significantly increased （P〈0.01）. Twenty-eight days after different treatments, compared with those in the untreated group, the learning and memory abilities of AD model rats in the piracetam, low-dose, medium-dose and high-dose NEK groups were significantly improved （P〈0.01 or P〈0.05）, and the expression of Aβ1-42 in the hippocampus decreased （P〈0.01 or P〈0.05）, and MMP-9 increased （P�

关 键 词：Naoerkang AIzheimer＇s disease amyloid beta-peptide matrix metalloproteinase-9 rats. 

分 类 号：R285[医药卫生—中药学]