激光治疗对噁唑酮诱导的溃疡性结肠炎大鼠细胞因子的影响  

Effects of laser therapy on cytokines in rats with oxazolone induced ulcerative colitis

作　　者：杜金刚[1] 刘畅格[2] 黄力平[2] 李猛[2] 韩玉山[1] 韩秀娟[1] 

机构地区：[1]天津市人民医院康复医学科,天津300121 [2]天津体育学院健康与运动科学系

出　　处：《中华物理医学与康复杂志》2011年第10期746-749,共4页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的探讨低能量激光治疗溃疡性结肠炎（UC）的分子机制，观察治疗前、后细胞因子肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和白细胞介素-10（IL-10）变化，为临床治疗提供依据。方法将健康成年雄性SD大鼠30只分为正常组（6只）、UC对照组（8只）、200mw激光治疗组（8只）和400mw激光治疗组（8只）。采用改良的噁唑酮致敏法制备大鼠UC模型。造模后对2个激光治疗组大鼠分别以功率为200mW与400mw的砷铝化镓半导体激光进行治疗，每次照射10min，每日1次，连续10d。治疗后将大鼠处死，酶联免疫吸附分析（ELISA）测定各组大鼠血清和组织匀浆液中TNF-α、IL-6、IL-10的含量。结果UC对照组大鼠与正常组比较，体重显著降低（P〈0．01），黏液脓血便，血清和结肠组织匀浆中TNF-α和IL-6含量显著升高（P〈0．05），IL-10显著下降（P〈0．01），造模成功。激光治疗后，大鼠体重和大便性状显著好转；400mW激光治疗组血清和结肠组织匀浆中TNF-α、IL-6显著降低（P〈0．01），IL-10显著提高（P〈0．05），接近正常水平；200mW激光治疗组血清中TNF-α和IL-6显著降低（P〈0．05），结肠组织匀浆中IL-6显著降低（P〈0．01），TNF-α降低不显著（P〉0．05），血清和结肠组织匀浆中IL-10提高没有达到显著性水平（P〉0．05）。结论400mW砷铝化镓半导体激光能够有效地双向调节噁唑酮诱导的UC大鼠细胞因子，减低致炎细胞因子，增加抗炎因子作用，可能是低能量激光治疗UC产生较优疗效的机制之一。Objective To explore the effects and molecular mechanisms of laser therapy on serum and colon tumor necrosis factor α （TNF-α） , interleukin-6 （IL-6） and interleukin-10 （IL-10） in rats with oxazolone induced ulcerative colitis （UC）. Methods Thirty adult male SD rats were randomly divided into four groups： a normal group （n=6）, a UC model group （n=8）, a400 mWlaser treatment group （n=8） and a200 mWlaser treatment group （ n = 8）. Odified oxazolone sensitization was used to induce UC models in the rats. The AsA1Ga semiconductor laser used in the treatment had a power of 200 mW or 400 mW. The therapy lasted for 10 days with daily 10 min sessions. The rats were sacrificed after treatment and enzyme-linked immunosorbent assay （ELISA） was used to measure IL- 6, IL- 10 and TNF-α in serum and ulcer tissues in all groups. Results Compared with the normal group, the weights of the UC model rats were significantly lower, and they had severe mucoid bloody stool. TNF-α and IL-6 levels in serum and colon tissues increased significantly and IL-10 decreased significantly, so the UC model was successfully established. After laser treatment, body weight gain and stool moderation in rats were observed, in the 400 mW group, and average TNF-α and IL-6 levels in both serum and colon tissue decreased significantly. IL-10 in- creased significantly, close to the level of the normal group. In the 200 mW group, serum TNF-α and IL-6 levels were significantly lower, and IL-6 in the colon tissue was reduced significantly. TNF-α did not lessen significantly, and serum and colon tissue IL-10 levels did not improve significantly compared with the model group. Conclusions 400 mW AsAIGa semiconductor laser irradiation can effectively regulate cytokines after oxazolone induced UC in rats in two ways. It reduces pro-inflammatory cytokines and increases the level of anti-inflammatory eytokines. This may imply one of the underlying molecular mechanisms of low energy laser treatment of UC.

关 键 词：溃疡型结肠炎 激光 细胞因子 

分 类 号：R285.5[医药卫生—中药学]