1,25-二羟基维生素D3对致死剂量脂多糖攻击的大鼠CD_4^+CD_(25)^+Treg细胞的调节作用  

Protective effects of 1,25-(OH)_2D_3 in rats given lethal doses of lipopolysaccharide

作　　者：祁晓平[1] 李培[1] 恽时峰[1] 黎介寿[1] 

机构地区：[1]南京大学医学院临床学院南京军区南京总医院全军普通外科研究所,江苏南京210002

出　　处：《中国现代普通外科进展》2011年第9期676-679,共4页Chinese Journal of Current Advances in General Surgery

基　　金：国家重点基础研究发展规划项目资助(2003cB515502)

摘　　要：目的:观察不同剂量1,25-二羟基维生素D3(1,25-(OH)2D3)短期应用对大鼠受致死剂量脂多糖(LPS)攻击后的保护作用,并探讨此保护作用是否与1,25-(OH)2D3对CD4+CD25+Treg细胞的调节作用有关。方法:将大鼠分成3个剂量组,每组20只。分别给予1,25-(OH)2D3 0.125μg/只、0.25μg/只、1μg/只灌胃,3次/周,共2周。另设对照组给予赋形剂。给药后第15天腹腔注射致死剂量LPS(10mg/kg),每组10只用于观察96 h内的死亡率;其余10只大鼠注射LPS 6 h后抽取外周血并留取脾脏标本。流式细胞仪检测大鼠外周血及脾脏CD4+CD25+Treg细胞数量,实时定量PCR检测脾脏Foxp3mRNA水平,ELISA检测外周血IL-10和TGF-β水平。结果:用1,25-(OH)2D3预处理的各组大鼠死亡率均显著低于对照组,用药各组外周血及脾脏CD4+CD25+Treg细胞数量、脾脏Foxp3mRNA表达水平、外周血IL-10及TGF-β水平也显著高于对照组。结论:1,25-(OH)2D3能够有效保护大鼠抵抗致死剂量LPS的攻击,这种保护作用可能与1,25-(OH)2D3上调CD4+CD25+Treg细胞的数量和功能有关。Objective： We evaluated the protective effects of 1,25-（OH）2D3 in rats given lethal doses of lipopolysaccharide and investigated whether such protection was related to CD4＋CD25＋Treg cells.Methods： 80 Inbred male Lewis rats were divided into 4 groups and treated with either 0.125 ug,0.25 ug,l ug of 1,25-（OH）2D3 or vehicle by gavage 3 times a week for 2 weeks.On day15,all rats were given lethal doses of lipopolysaccharide（LPS,10mg/kg,i.p.） and the mortality of 10 rates of each group in were recorded within a 96 hour period.Six hours after LPS injection,peripheral blood and spleen tissue of the remaning rats were taken to count CD4＋CD25＋Treg cells by flow cytometry.Expression of Foxp3mRNA in spleen was measured by real-time PCR method.Results： The mortality rates of rats pre-treated with 1,25-（OH）2D3 were significantly lower than the control group.The number of CD4＋CD25＋Treg cells,the expression of Foxp3mRNA in spleen,and IL-10 and TGF-βlevels in the peripheral blood in these groups were Significantly higher than the control group.Conclusion： 1,25-（OH）2D3 can effectively protect rats from a lethal dose of LPS.This protection may be related to the up-regulation of the number and function of CD4＋CD25＋Treg cells by 1,25-（OH）2D3.

关 键 词：1 25-二羟基维生素D3 调节性T细胞 免疫调控 脂多糖 

分 类 号：R392[医药卫生—免疫学]