超声生物显微镜成像对载脂蛋白E基因敲除小鼠早期动脉粥样硬化的分析  被引量:2

Analysis of early-stage atherosclerosis in apolipoprotein-E knockout mice by ultrasound biomicroscopy

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作  者:杨娅[1] 王艳红[1,2] 谢谨捷[1,3] 李嵘娟[1] 李治安[1] 王征[1] 宋砾[1] 罗向红[1] 苏瑞娟[1] 秦彦文[4,5] 

机构地区:[1]首都医科大学附属北京安贞医院超声科,北京市100029 [2]首都医科大学附属北京友谊医院 [3]北京市朝阳区第二医院 [4]首都医科大学附属北京安贞医院 [5]北京市心肺血管疾病研究所心血管生物研究室

出  处:《临床超声医学杂志》2011年第10期653-655,共3页Journal of Clinical Ultrasound in Medicine

基  金:国家自然科学基金(30672000);北京市自然科学基金(7102044;7082020)

摘  要:目的利用超声生物显微镜(UBM)成像分析载脂蛋白E基因敲除(ApoE-/-)小鼠主动脉早期动脉粥样硬化(AS)的超声特征。方法 8周龄和16周龄雄性ApoE-/-小鼠各8只为实验组,同周龄的雄性C57BL/6小鼠各8只为对照组。使用UBM观察小鼠主动脉根部、升主动脉和主动脉弓的形态结构,并于主动脉根部短轴切面测量内-中膜厚度(IMT)。检查后处死小鼠取标本,行病理学检查。结果 ApoE-/-小鼠的主动脉根部IMT较同周龄对照组增厚,差异有统计学意义(P<0.01);16周龄ApoE-/-小鼠较8周龄ApoE-/-小鼠主动脉根部IMT增厚更为明显,差异有统计学意义(P<0.01)。形态学病理检查显示6只16周龄ApoE-/-小鼠主动脉根部、升主动脉小弯侧及无名动脉可见局部管壁增厚,有散在白色脂纹形成。结论 UBM成像可无创观察活体ApoE-/-小鼠升主动脉早期AS的病变特征,能有效监测ApoE-/-小鼠AS病变的进程。Objective To analyze the ultrasound characteristics of the early-stage atherosclerosis (AS) of aorta in apolipoprotein-E knockout ( ApoE - / - ) mice by ultrasound biomicroscopy (UBM). Methods Eight male 8-week mice ApoE -/- mice and 8 male 16-week ApoE -/- mice were used as experimental group, and age-matched and week-matched male C57BL/6 mice were used as control group. The morphology of the aortic root, the ascending aorta and the aortic arch were observed by UBM, and the intima-media thicknes (IMT) was measured at the aortic root short-axis view. Then the pathological examinations were taken. Results The IMT of aotic root in ApoE-/- mice was thicker than that of control group (P 〈 0.01 ) , the IMT of 16-week were thicker than that of 8-weeks ApoE-/- mice (P 〈 0.01 ). The morphology pathlogical examination showed there were white fatty streaks in the walls of aortic root, lesser curvature side of ascending aorta and innominate artery in six 16-week ApoE-/- mice, and the walls became thicker. Conclusion UBM can non-invasively observe the characteristics of earlystage ascending aorta AS in ApoE - / - mice in vivo, it can be used to efficiently monitor the progression of AS in ApoE - / - mice.

关 键 词:超声生物显微镜 动脉粥样硬化 小鼠 载脂蛋白E 

分 类 号:R543.1[医药卫生—心血管疾病] R445.1[医药卫生—内科学]

 

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