亚热带木腐真菌产漆酶及其酶学性质  

Production and Enzymatic Properties of Laccase from a Subtropical Wood-Rot Fungus

作　　者：傅恺[1] 付时雨[1] 张丽[1] 周攀登[1] 詹怀宇[1] 

机构地区：[1]华南理工大学制浆造纸工程国家重点实验室,广东广州510640

出　　处：《华南理工大学学报（自然科学版）》2011年第9期152-157,164,共7页Journal of South China University of Technology(Natural Science Edition)

基　　金：国家自然科学基金资助项目(30771689);广东省科技计划项目(2007B031600002)

摘　　要：对选育的一株木腐真菌Psathyrella candolleana进行液体深层发酵生产漆酶,通过硫酸铵盐析和离子交换色谱等纯化技术分离发酵酶液后,得到纯度较高的漆酶,并对其酶学性质进行研究.结果表明:P.candolleana在发酵罐中的漆酶产量最高可达9100U/L;分离纯化后得到漆酶活性组分,其比活力达到438.0 U/mg,纯化倍数为13.2,得率为50.1%;该漆酶蛋白分子质量约为61.2 ku,米氏常数为16μmol/L;该漆酶催化反应最适温度和pH值分别为45℃和5.0,在4～10℃和pH 9.0的条件下漆酶活力稳定性较好,Mg2+、Mn2+、Zn2+等金属离子对其活力的影响较小.Laccase from a newly isolated wood-rot fungus Psathyrella Candolleana, was produced via the submerged fermentation, and high-purity laccase was obtained by means of the ammonium sulfate precipitation and the ion-exchange chromatography. Then, the enzymatic properties of the laccase were also analyzed. The results indicate that the maximal activity of the laccase from P. candolleana in the fermentor reaches 9100 U/L, that the active component of the lacease achieved after 13.2-fold purification is of a specific activity of 438.0 U/rag with a yield of 50. 1%, that the molecular mass and Michaelis constant of the laccase are respectively about 61.2ku and 16 μmol/L, and that the optimal pH value and temperature for the laccase activity are 5. 0 and 45 ℃, respectively. Moreover, it is also found that the laccase activity is stable under 4 - 10℃ at pH 9.0 and is not sensitive to Mg2＋, Mn2＋ and Zn2 ＋.

关 键 词：白黄小脆柄菇 白腐菌 漆酶 纯化 漆酶活力 稳定性 

分 类 号：Q814[生物学—生物工程]