金黄益胆颗粒对大鼠重症急性胰腺炎肝损伤的保护作用  

Protective effect of Jinhuangyidan Granules on liver injury in rats with severe acute pancreatitis

作　　者：赵立新[1] 马向明[2] 曹立瀛[2] 刘四清[2] 付庆江[2] 张青松[3] 

机构地区：[1]河北联合大学研究生院,河北省唐山市063000 [2]河北联合大学附属开滦医院肝胆外科,河北省唐山市063000 [3]河北联合大学附属开滦医院普外科,河北省唐山市063000

出　　处：《世界华人消化杂志》2011年第25期2654-2659,共6页World Chinese Journal of Digestology

基　　金：国家科技支撑基金资助项目;No.2006BAI04A15~~

摘　　要：目的:探讨金黄益胆颗粒(jinhuangyidan granules)对大鼠重症急性胰腺炎肝损伤的保护作用和可能机制.方法:54只♂SD大鼠随机分为假手术组(sham operation group,SO)、重症急性胰腺炎组(severe acute pancreatitis,SAP)和金黄益胆颗粒治疗组(jinhuangyidan granules treatment,JHT),每组分为2、6、12h三个时间点组,每个时间点组6只.5%的牛黄胆酸钠逆行胰胆管注射制备SAP模型.各组于造模后2、6、12h处死大鼠,检测血清淀粉酶(serumamylase,AMY)、谷丙转氨酶(ALT)、谷草转氨酶(AST),计算肝脏系数,HE染色观察胰腺、肝脏组织病理,酶联免疫吸附试验(ELISA)方法检测TNF-α、IL-6、ET-1水平.免疫组化检测肝脏组织中核转录因子-κB的活性.结果:各时间带点SAP组与SO组比较,AMY、ALT、AST、TNF-α、IL-6、ET-1均升高,肝脏系数在6h、12h差异有统计学意义(P<0.05);在6h、12hJHT组与SAP组比较,AMY、肝脏系数、ALT、AST、TNF-α、IL-6、ET-1指标均明显降低(P<0.05).6h时间点SO组、SAP组和JHT组NF-κB表达分别为(5.32±0.24vs49.89±3.53vs19.18±1.51),三组之间差异有统计学意义(P<0.05);12h时间点各组之间NF-κB表达分别为(6.38±0.24vs54.73±1.68vs31.15±2.25),三组之间差异有统计学意义(P<0.05).胰腺和肝脏的病理镜下观察水肿和炎性介质表达JHT组较SAP组明显改善.结论:金黄益胆颗粒可以一定程度的改善大鼠重症胰腺炎肝脏的损伤,改善微循环,其对大鼠重症急性胰腺炎肝损伤的保护作用机制可能与抑制NF-κB的活性进而下调TNF-α、IL-6的表达有关.AIM： To investigate the protective effect of Jinhuangyidan Granules on liver injury in rats with severe acute pancreatitis （SAP） and to explore the possible mechanisms involved.METHODS： Fifty-four male Sprague-Dawley （SD） rats were randomly divided into three groups： sham operation group, SAP group and Jinhuangyidan Granules group. Each group wasfurther divided into three subgroups for testing at different time points after treatment （2, 6 and 12 h）. SAP was induced by retrograde injection of 5% sodium taurocholate into the biliary pan- creatic duct. Serum amylase （AMY）, ALT, AST and the liver coefficient were measured. The levels of TNF-α, IL-6 and ET-1 were examined by ELISA, and the expression of NF-κB in the liver was evaluated by immunohistochemistry.RESULTS： Compared to the sham operation group, the levels of AMY, ALT, AST, TNF-α, IL-6, ET-1 and NF-KB increased significantly in the SAP group. The liver coefficient also differed significantly between the sham operation group and SAP group at 6 and 12 h （both P 〈 0.05）. Compared to the SAP group at 6 and 12 h, the levels of AMY, ALT, AST, TNF-α, IL-6, ET-1 and NF-KB decreased significantly in the Jinhuangy- idan Granules group （all P 〈 0.05）. At 6 h, the ex- pression of liver NF-κB was significantly higher in the SAP group than in the sham operation group at 6 and 12 h （6 h： 49.89 ± 3.53 vs 5.32 ± 0.24; 12 h： 54.73 ± 1.68 vs 6.38 ± 0.24, both P 〈 0.05）, but was significantly lower in the Jinhuangyidan Granules group than in the SAP group （6 h： 19.18 ± 1.51 vs 49.89 ± 3.53; 31.15 ± 2.25 vs 54.73 _± 1.68, both P 〈 0.05）. Compared to the SAP group, the levels of edema and inflammatory mediators ameliorated significantly in the Jinhuangyidan Granules group.CONCLUSION： Jinhuangyidan Granules amelio- rates liver injury and improve microcirculation in SAP rats possibly by inhibiting NF-κB activity and down-regulating inflammatory mediators.

关 键 词：金黄益胆颗粒 胰腺炎 肝损伤 核转录因子-ΚB 炎症介质 

分 类 号：R285.5[医药卫生—中药学]